Chugai Seiyaku Kabushiki Kaisha

Norimichi Ochi

Kozo Takeda

Manabu Matsuhashi

Kimie Ishii

Akinori Imamura

A method for manufacturing an antibody formulation in which DNA contaminants are removed by binding the antibody to a protein-A or probtin-G affinity column and eluting the antibody with an acidic eluting solution, preferably of low conductivity.

Method for removing viruses in a physiologically-active protein-containing sample

Protein purification method

A method is provided for purifying physiologically active proteins, especially antibodies, in order to remove impurities such as DNA contaminants and viruses with minimal loss of physiologically active proteins. The physiologically active protein is introduced into an aqueous solution of low conductivity at a pH of below the isoelectric point of the physiologically active protein to precipitate impurities as particles. The particles are removed, leaving a purified physiologically active protein.

Method for removing DNA contaminants from a protein-containing sample

A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:

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Tokyo, Japan

Norimichi Ochi