National Institute of Advanced Industrial Science and Technology

Hokkaido University

Hokusan Co. Ltd

The Hokuren Federation of Agricultural Cooperatives

School Juridical Person the Kitasato Institute

Noriko Itchoda

Takeshi Matsumura

Akira Ito

Chikara Masuta

Toru Gotanda

Noriko Tabayashi

Uiko Kagaya

Takeaki Ishihara

Hanae Sasaki

Hiromi Ikadai

Takashi Aoki

Noriho Fukuzawa

It is an object of the present invention to provide a transmission-blocking vaccine using an immunogenic protein which is specifically expressed in the oocyst stage of malaria parasites or a peptide fragment thereof, and an oral transmission-blocking vaccine capable of immunizing various animals involved in the malaria infectious cycle with such a vaccine. The present invention relates to a malaria transmission-blocking vaccine for oral administration containing an immunogenic protein derived from malaria parasite which is specifically expressed in the oocyst stage of malaria parasites or a peptide fragment thereof, and a method of blocking the transmission of malaria using the same.

Malaria transmission blocking vaccine

The object of the present invention is to provide a therapeutic agent illustrating an immediate and sustained effect on chronic intractable external otitis. The present invention provide a composition, pharmaceutical composition, food and feed for preventing and/or treating chronic intractable external otitis, each comprising interferon-&#x3b1; (IFN&#x3b1;) as an active ingredient. Orally administering the composition of the present invention can provide inhibition, alleviation, treatment and prevention for chronic intractable external otitis. According to the present invention, IFN&#x3b1; can give a sufficient effect even when administered in a very small amount.

Therapeutic effect of oral interferon a administration on chronic intractable external otitis

The present invention is to provide a novel non-diffusing plant virus vector wherein virus vector infection and proliferation are possible only in a recombinant plant transformed with a gene necessary for viral proliferation, thereby enabling avoidance of unintended diffusion of a recombinant virus, a selective and specific expression system therefor, and a method of expression thereof which comprises combining a non-diffusing virus vector lacking a gene involved in intercellular movement of a cucumber mosaic virus (CMV) genome and a transgenic plant transformed with the lacked gene involved in intercellular movement for the non-diffusing virus vector to establish infection and proliferation selectively and specifically in the transgenic plant.

Non-diffusing plant virus vector

A novel plant virus vector and a method of gene expression into plants are provided, and the present invention relates to a plant virus vector characterized in that a part of the sequence of the 2b region of the RNA2 molecule of the Cucumber mosaic virus (CMV) is deleted; the aforementioned plant virus vector characterized in that the region from the StuI site to the stop codon of the 2b ORF of pCY2, which is an infectious clone of CMV-Y RNA2, is deleted; and a method of gene expression in plants characterized in that a foreign gene is introduced so as to be stably expressed in a plant using the genetic recombinant. This vector is useful as a plant RNA vector for causing stable expression of foreign genes.

Plant virus vector

Growing community of inventors

Hokkaido, Japan

Noriko Itchoda