Kyushu University

Hitachi, Ltd.

Hitachi Aloka Medical, Ltd.

Other

The University of Tokushima

Noriho Kamiya

Kounosuke Hayashi

Kenji Nagai

Masahiro Goto

Sumihare Noji

Kyoichi Matsuba

Yoshiro Tahara

Kosuke Moriyama

Yoshiyuki Hiraishi

Kosuke Minamihata

Rie Wakabayashi

Kensuke Yahiro

Provided is a method for producing a hydrogel, which enables a hydrogel comprising polyethylene glycol to be produced under low peroxidase concentration conditions and physiological conditions. The method for producing a hydrogel involves crosslinking polyethylene glycol having two or more thiol groups using peroxidase in the presence of a phenol compound.

Method for producing hydrogel, method for enveloping envelopment target, and method for releasing envelopment target

A fusion protein for protein detection in which are fused: a protein domain including at least one among a C1 domain of protein G, a C2 domain of protein G, and a C3 domain of protein G; and a double mutant D153G/D330N ofalkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by Gly and the 330 amino acid residue Asp has been substituted by Asn, a double mutant D153H/D330N ofalkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by His and the 330 amino acid residue Asp has been substituted by Asn, or a double mutant K328R/D330N ofalkaline phosphatase (BAP) in which the 328 amino acid residue Lys has been substituted by Arg and the 330 amino acid residue Asp has been substituted by Asn.

Fusion protein for protein detection, and method for detecting protein

Provided is a protein-polymer complex which is capable of detecting a target with good sensitivity. Specifically provided is a protein-polymer complex comprising a polymer having a glutamine (Gln) residue or a primary amine on a side chain, wherein either a protein having a primary amine is bound to the glutamine (Gln) residue, or a protein having a glutamine (Gln) residue is bound to the primary amine.

Protein-polymer complex, TGase substrate-containing polymer, TGase substrate-containing monomer, method for producing protein-polymer complex, and method for improving protein function at solid-liquid interface or in vicinity of solid-liquid interface

Provided is a method for producing a nucleic acid probe that can detect a target substance with good sensitivity. A method for producing a nucleic acid probe, comprising: a 3'-terminal addition step of adding at least one nucleoside triphosphate derivative having a glutamine (Gln) residue or a lysine (Lys) residue to the 3&#x2032;-terminal of a nucleic acid using a 3&#x2032;-terminal addition enzyme which adds a nucleotide to the 3&#x2032;-terminal of a nucleic acid, and a labeling compound binding step of either binding a labeling compound having a lysine (Lys) residue and containing a labeling moiety to the glutamine (Gln) residue using a transglutaminase (TGase), or binding a labeling compound having a glutamine (Gln) residue and containing a labeling moiety to the lysine (Lys) residue using a transglutaminase (TGase).

Method for producing protein-nucleic acid conjugate, and method for detecting target substance

An object of the present invention is to provide a drug delivery carrier that is free from the drug leakage problem and has an easily controllable particle size, and that can be used to deliver water-soluble drugs such as genes and proteins in a wide range of applications, including delivery of water-soluble drugs that do not have high anionic properties, and also can be used as a non-viral gene vector. The invention also provides a process for production of such drug delivery carriers. The drug delivery carrier of the present invention includes a water-soluble drug double-coated with two types of inner and outer surfactants 1 and 2.

Water-soluble drug carrier and process for producing the same

A novel nucleoside triphosphate derivative, a nucleic acid probe, and a multilabeled nucleic acid probe that can detect a target nucleic acid conveniently and with high sensitivity, as well as a method for producing the multilabeled nucleic acid probe, and a method for detecting a target nucleic acid using the multilabeled nucleic acid probe or the nucleic acid probe. A target nucleic acid can be detected conveniently and with high sensitivity by using a transglutaminase (TGase), and by using a multilabeled nucleic acid probe in which a plurality of labeling portions have been introduced in advance by covalent binding, or by introducing a plurality of labeling portions by covalent binding into a nucleic acid probe that has been hybridized with the target nucleic acid.

Nucleoside triphosphate derivative, nucleic acid probe, multilabeled nucleic acid probe, method for production of multilabeled nucleic acid probe, and method for detection of target nucleic acid

Growing community of inventors

Fukuoka, Japan

Noriho Kamiya