Toyota Jidosha Kabushiki Kaisha

Kabushiki Kaisha Toyota Chuo Kenkyusho

Okayama Prefecture

The University of Tokyo

Riken Corporation

Meiji University

Norihiro Mitsukawa

Chikara Ohto

Nobuhiko Muramoto

Masaru Takagi

Kyoko Matsui

Satoshi Kondo

Tomotsugu Koyama

Nobutaka Mitsuda

Hiroki Sugimoto

Hiroshi Chatani

Tomoko Tanaka

Yukio Okamura

Kenichi Ogawa

Madoka Yonekura

Kunihiro Ohta

Ritsuko Yogo

Hidenori Tanaka

Etsuko Hattori

Takahiro Nakamura

Satoshi Katahira

Akinori Ikeuchi

Makoto Hayashi

Risa Nakamura

Kazufumi Tabata

Kazuto Kugou

Shuichi Osato

Shuichi Ohsato

Matsui Kyoko

Masuru Takagi

Masaru Tagaki

A method of inducing genetic recombination, including: allowing a protein having DNA double-stranded cleavage activity to act in cells of a eukaryotic organism which is a polyploidy inherently possessed by a eukaryotic organism. In eukaryotic organisms, various genetic recombination generates new genome set composition. This is done to obtain a population of eukaryotic organisms that hold the modified genomic set.

Method of inducing genetic recombination, and use therefor

A method of modifying the genome of an organism, wherein the modification method includes modifying the genome of the organism by using in a cell of the organism a protein having an optimal temperature for double-stranded DNA breakage activity in an ordinary temperature region.

Method of modifying genome of organism and use thereof

This invention is intended to increase biomass production through suppression of expression of a gene functioning in relation to intracellular vesicular transport or inhibition of functions of a protein encoded by such gene. To this end, expression of a gene encoding the coatomer adapter zeta subunit is suppressed, the coatomer adapter zeta subunit is inhibited, expression of a gene encoding the clathrin adaptor small (sigma) subunit is suppressed, or the clathrin adaptor small (sigma) subunit is inhibited.

Plant in which expression of a clathrin and/or coatomer subunit is suppressed or inhibited, a method for increasing the production of plant biomass and a method for producing a plant

A technique by which the production of plant biomass can be significantly increased is provided. A protein phosphatase 2C gene having 3 consensus sequences comprising the amino acid sequences shown in SEQ ID NOS: 1-3 from the N-terminal side in such order is over-expressed.

Gene for increasing the production of plant biomass and/or seeds and method for use thereof

A gene having novel functions is searched for, by which plant weight (that is, biomass level) can be increased and by which substance productivity can be increased or decreased. A chimeric protein is expressed in which a transcriptional factor comprising the amino acid sequence shown in SEQ ID NO: 2, 4, or 6 is fused to a functional peptide that converts an arbitrary transcriptional factor into a transcriptional repression factor.

Gene for increasing plant weight and method for using the same

According to the present invention, a gene having a novel function that can cause an increase or decrease in seed protein content is searched for. A chimeric protein obtained by fusing a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 1 to 76 and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor or a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 77 to 84 is expressed in a plant.

Plant with reduced protein productivity in seeds and method for producing same

A method of increasing the biomass of a plant that includes planting a plant having plant cells carrying an exogenous gene that encodes a thermophilic restriction enzyme that promotes double-stranded DNA breakage, and growing the plant at least until after true leaf development, wherein the mean biomass of plants having the plant cells carrying the exogenous gene that are grown at least until after true leaf development is increased in comparison with the mean biomass of plants of the same species that do not carry the exogenous gene that are grown for the same amount of time.

Method for increasing plant biomass using an exogenous gene encoding a thermophilic restriction enzyme

A method for producing a plant includes growing a plant from plant cells in which a promoter and a coding sequence under the control of the promoter have been artificially introduced; and selecting the plant in which an amount of phloem tissue is increased 2.0 times or more in comparison with a wild type plant of the same species. The promoter is a promoter for a gene encoding a protein having at least 60% identity with the amino acid sequence of tracheary element differentiation inhibitory factor (TDIF) receptor (TDR) of. The coding sequence encodes either (i) a protein having three common sequences composed of the amino acid sequences of SEQ ID NOS: 1 to 3 in this order starting from the N-terminal side, or (ii) a protein having 95% or more identity with the amino acid sequence of SEQ ID NO:5.

Plant suitable for increasing production of phloem tissue and use of same

A technique by which the production of plant biomass can be significantly increased is provided. A gene encoding protein phosphatase 2C having 3 consensus sequences comprising the amino acid sequences shown in SEQ ID NOS: 1-3 from the N-terminal side in such order and a gene encoding glutathione-binding plastid-type fructose 1,6-bisphosphate aldolase is introduced, or an expression control region of endogenous genes corresponding to the genes are modified.

According to the present invention, environmental stress resistance is imparted to a plant or the environmental stress resistance of a plant is improved. At least one gene selected from the group consisting of an LRR-RLP gene selected from a 1st group (including At2g33080), an LRR-RLK gene selected from a 2nd group (including At1g69990), and an LRR-RLK gene selected from a 3rd group (including At5g39390) is introduced into a plant, or an expression control region of an endogenous gene is altered in a plant.

Gene capable of imparting environmental stress resistance to plants and method for utilizing the same

An object of the present invention is to search for a gene having a novel function that can cause an increase or decrease in material productivity, and particularly, fat and oil content. In the present invention, a chimeric protein obtained by fusing a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 1 to 158 and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor is expressed in a plant.

Gene capable of improving material productivity in seed and method for use thereof

Growing community of inventors

Miyoshi, Japan

Norihiro Mitsukawa