National Institute of Advanced Industrial Science and Technology

Director-general of Agency of Industrial Science and Technology

Reqmed Company, Ltd.

Norihiko Minoura

Tadashi Matsumoto

Seiichi Aiba

Yukihiko Fujiwara

Alexandre Rachkov

Hirotaka Uzawa

Hu Minjie

Hiroki Itou

Kaori Endo

Kazuhiro Taguchi

The methods for producing macromolecule identifying polymers according to the present invention comprise the steps of polymerizing a starting monomer in an aqueous solution in the presence of a macromolecule, a crosslinking agent, and a radical polymerization initiator to produce a polymer containing the macromolecule in its interior; and removing the macromolecule from the polymer containing the macromolecule to thereby produce the macromolecule identifying polymer having a molecular imprint of the macromolecule. In this method, the crosslinker has a solubility in water at 25&#xb0; C. of 100% by mass or higher.

Methods for producing macromolecule-identifying polymers

An objective of the present invention is to provide ionic polymers that can provide for polymer-comprising substrates whose surfaces can stably and easily immobilize ligands such as sugar chains interacting with biological substances, in which the ligand portion exists stably even in water or buffer, and its detection capability does not decrease for a long period of time; and substrates comprising the polymers. The ionic polymer of this invention comprises two or more ionic functional groups (A) and two or more biointeracting groups represented by formula L-X-B-[wherein, L denotes a ligand capable of interacting with a biological substance, X denotes a spacer, and B denotes a binding group] as side chains covalently bound to a main chain polymer.

Ionic polymers and polymer-comprising substrates

Cells such as mammalian cells are cultivated on a bed of a solid material whose surface is formed of a specific polymer or copolymer of an amino acid, i.e. a poly(methionine) or a copolymer of .gamma.-benzyl glutamate.

Culture of cells

Growing community of inventors

Ibaraki, Japan

Norihiko Minoura