Medinet Co., Ltd.

Norihiko Hata

Hidemasa Jinguji

Naoko Ariyoshi

Shiho Sato

Kazutoshi Sato

Atsutaka Noguchi

Akihiro Hosoi

There is provided a cell culture evaluation system, a cell culture evaluation method, and a cell culture evaluation program which are capable of estimating and evaluating the lag time or the minimum doubling time and objectively and adequately determining whether or not a cell population is stimulated for proliferation by using an average projected area of a cultured cell population or the rate of increasing the ratio of the non-single-cells as an evaluation parameter when culturing the cells. Images of the cell population to be cultured statically are acquired in a culture vessel, the average projected areas of the cells are calculated from the images for the respective culture times, and the lag times at lag phase are calculated from the calculated average projected areas of the cells. The single-cells and the non-single-cells are discriminated from the images, and the increasing rate of the non-single-cells is calculated from the ratio of the non-single-cells in the cell population to determine the minimum doubling time from the increasing rate of the non-single-cells. Whether or not the cell population is stimulated for proliferation is determined from the ratio of the non-single-cells.

Cell culture evaluation system for measuring suspension cells, cell culture evaluation method for measuring suspension cells, and cell culture evaluation program for measuring suspension cells

The invention intends to provide a cell culture shaking device which is able to improve the efficiency of cell culture while preventing the cells from becoming damaged. The shaking devices used in cell culture apparatuses for culturing cells in flexible culture bags for storing culture suspension having cells inoculated therein includes: shaking mechanisms having operating panels for pressing the culture bags repeatedly, so that the culture suspension in the culture bags is stirred by being pressed by a plurality of projections projecting for the operating panels.

Cell culture shaking device and shaking culture method as cell culture method

The invention intends to provide a cell culture apparatus which is able to realize an adequate culture according to the culture state of cells while alleviating the labor of an operator. The cell culture apparatus includes a culture bag for causing the cells to proliferate, a cell inoculation cassette (or culture bag as an antibody stimulating and proliferation culture vessel) for stimulating the cells by an inducer for the proliferation, a culture medium cassette for storing a culture medium supplied to the culture bag and the cell inoculation cassette, a CCD camerafor acquiring images of the cells in the cell inoculation cassette, and an image processing computer and an operation control computer for determining the culture state (proliferation capability and proliferation ability of the cells) of the cells from the images of the cells acquired by the CCD camera, and causing a culture operation to be carried out on the basis of the determination.

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Yokohama, Japan

Norihiko Hata