Kikkoman Corporation

Noriaki Hattori

Seiji Murakami

Keiko Yajitate

Motoo Nakajima

Tatsuya Sakakibara

Kazuhiro Imai

Teruo Watarai

Toshinori Igarashi

Moto-o Nakajima

Morisaku Saito

The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.

Luciferase and methods for measuring intracellular ATP using the same

Luciferase and methods for assaying intracellular ATP by using the same

A wipe inspecting instrument comprising a sample collecting member and a reactive reagent container. The sample collecting member comprises a tubular main body having both upper and lower ends thereof opened, a sample wiping member removably fitted to the upper open portion of the main body and an extracting liquid container disposed below the main body. The sample wiping member includes a retaining member inserted into the upper part of the main body and a cotton swab held by the retaining member. Further, the sample collecting member includes a splash-proofing member disposed at a lower part of the extracting liquid container, for preventing the splash of the extracting liquid. The reactive reagent container has a test tube shape and so inserted into the open portion at the lower end of the main body as to be capable of moving up and down.

Sample collecting member and wiping inspection instrument

Method for analyzing intracellular components

The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.

ATP eliminator and the process for determining biological cells

There are provided a method and a kit for testing microbial drug resistance and a method and a kit for measuring minimum inhibitory concentration for a microorganism, in which highly reliable evaluation results are obtained by preventing false evaluation based on false-resistance of microorganisms to a drug. The method of testing microbial drug sensitivity comprises inoculating and culturing a microorganism in a drug-containing medium to obtain a medium containing the microorganism morphologically transformed by the influence of the drug, adding an ATP elution agent and an ATP deletion agent to the culture to delete the eluted ATP and other ATP in the medium, and comparing the amount of ATP in the microorganism remaining in the culture with the amount of ATP in the microorganism separately cultured in a medium not containing said drug and treated in the same manner as above. The method of measuring minimum inhibitory concentration for a microorganism is carried out in the same manner as above.

Method and kit for testing microbial drug sensitivity, and method and

The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme selected from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.

Growing community of inventors

Noda, Japan

Noriaki Hattori