Eastman-Kodak Company

Johnson & Johnson Clinical Diagnostics, Inc.

Norbert S Norkus

Harold C Warren

Margaret J Smith-Lewis

Randall S Johnson

Kerinchan P Babaoglu

Angela H Reifler

An aqueous wash solution is buffered to a pH of from about 5 to about 9 and contains at least about 1.5 weight percent of a compound comprising a lower alcohol sulfate anion having from 6 to 10 carbon atoms and an alkali metal or ammonium cation, such as sodium decyl sulfate. This wash solution is useful in a method for the determination of an immunological ligand, and is not prone to crystallization at lower temperatures. Particularly, it is useful for washing the immunological complex formed between the ligand and a receptor molecule therefor. Unreacted materials can be readily separated from the complex by the washing, particularly if the separation is carried out using a filtration membrane in a test device. A test kit for ligand determination comprises the wash solution as well as one or more receptors for the ligand, at least one of which is labeled for detection. This kit is particularly useful for measuring human chorionic gonadotropin (hCG) as an early indicator of pregnancy.

Lower alcohol sulfate wash solution

Immunoassay kit for analysis of biological liquids

A method of using a pipette comprising a flexible tube portion having an integrally formed squeezable bulb portion, a spring clip arranged between the ends of the pipette to inhibit air from being aspirated into the pipette and a filter at the inlet end of the pipette.

Method of using a pipette

Lower alcohol sulfate wash solution, test kit and method for the

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Webster, NY, United States of America

Norbert S Norkus