Sandoz Ag

Biochemie Gesellschaft M.b.h

Norbert Palma

Franz Knauseder

Jörg Windisch

Günter Stempfer

Kurt Schoergendorfer

Hans Boehling

Waander Riethorst

Arno Reichert

Peter Alliger

The present invention relates to the preparation of a recombinant polypeptide, which polypeptide upon expression has been secreted into the periplasm of a transformed host cell. In particular, this invention relates to a process to enhance the extraction yield of the recombinant polypeptide from the periplasm before further downstream processing.

Process for the preparation of recombinant polypeptides

The present invention relates to an expression vector, comprising a polynucleotide encoding a fusion protein comprising the signal sequence of the gac gene ofand a polypeptide of interest, a prokaryotic host cell transformed with such an expression vector and a process for production of a polypeptide of interest using said host cell and said expression vector.

Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides

A process is presented for producing spherical particles containing microorganism cells having desired enzyme activity. The process comprises the steps of mixing the cells directly with a primary or secondary amine-containing polymer, combining the resulting mixture with an organic solvent to form a two-phase system, and then adding a bifunctional cross-linking agent to yield the spherical particles. The preferred enzyme activities are D-amino acid oxidase and glutarylacylase activities.

Spherical particles containing microorganism cells having enzyme activity

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Breitenbach a. Inn, Austria

Norbert Palma