Eli Lilly and Company

Norbert Neuss

Robert L Hamill

Walter M Nakatsukasa

Gary G Marconi

Donald R Brannon

Susan S Tafur

Ian G Wright

Roger D Miller

Albert J Barnes

Deoxynarasin antibiotic complex, comprising 20-deoxynarasin and 20-deoxy-epi-17-narasin, is produced by submerged aerobic fermentation of Streptomyces aureofaciens NRRL 11181. 20-Deoxynarasin and 20-deoxy-epi-17-narasin are separated and isolated by chromatography. The deoxynarasin complex, 20-deoxynarasin and 20-deoxy-epi-17-narasin are antibacterial and anticoccidial agents and also increase feed-utilization efficiency in ruminants.

Process for producing deoxynarasin antibiotics

An apparatus is described which is adaptable for use in coordinating UV/VIS absorbency values of a chromatography eluate containing antimicrobial constituents with bioautograph test results. Such an apparatus is comprised of a UV/VIS spectrophotometer equipped with a continuous recording monitor, a pair of parallel metering valves, a stream splitter, a rotatable drum adapted to hold a piece of absorbent material and means to rotate said drum. The method involves the use of such an apparatus in combination with the bioautograph test to coordinate said absorbency valves with the test results from a plurality of bioautograph test organisms.

Apparatus and method for coordinating chromatographic separation (HPLC)

Deoxynarasin antibiotic complex, comprising 20-deoxynarasin and 20-deoxy-epi-17-narasin, is produced by submerged aerobic fermentation of Streptomyces aureofaciens NRRL 11181. 20-Deoxynarasin and 20-deoxy-epi-17-narasin are separated and isolated by chromatography. The deoxynarasin complex, 20-deoxynarasin and 20-deoxy-epi-17-narasin are antibacterial and anticoccidial agents and also increase feed-utilization efficiency in ruminants. 20-Deoxynarasin and its salts are cardiotonic agents.

Deoxynarasin antibiotics

4-Desacetoxy-4-oxovinblastine, prepared by oxidation of 4-desacetylvinblastine, useful as an intermediate.

4-Desacetoxy-4-oxovinblastine

Vindoline and vinblastine demethylated by submerged culture fermentation with S. albogriseolus.

Des-N-methylvindoline

Leurosine is converted to vincathicine by treatment with acid.

Conversion of leurosine to vincathicine

N-demethylation of indole alkaloids by S. albogriseolus

4-Desacetoxyvinblastine, a novel indole-dihydroindole alkaloid obtained in small quantities from Vinca rosea, intermediate for the preparation of antimitotic compounds.

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Indianapolis, IN, United States of America

Norbert Neuss