Atto-tec Gmbh

Karl-heinz Drexhage

Norbert Kemnitzer

Karl-Heinz Drexhage

Jutta Arden-Jacob

Alexander Zilles

Monika Hamers-Schneider

The invention relates to compounds of the general formulae (I)-(IV), which are characterized by substituents B comprising one or more sulfonic acid groups and their use as marker groups for the detection of analytes.

Polysulfonated fluorescence dyes

The present invention relates to polycyclic compounds of the general formula (I) which are distinguished by an amine substituent NRRon the central carbon atom of the chromophore, to processes for their preparation, and to the use thereof for the determination of analytes.

Amine-substituted tricyclic fluorescent dyes

The invention concerns the production of quinoline compounds containing sulfonic acid groups, the said quinoline compounds and their conversion into dyes containing sulfonic acid groups. The dyes according to the invention are used especially to label analytes, for example to label biomolecules.

Sulfonamide derivatives of polycyclic dyes used for analytical applications

The present invention relates to carboxamide-substituted dyes, the production and use of such dyes as labeling groups in analytics.

Carboxamide-substituted dyes for analytical applications

A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having structure (I) wherein Y and Y are individually hydroxyl, amino, imminium, or oxygen, R -R are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinatios thereof, and R is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having general structure (II) where substituents R -R correspond to like-referenced substituents in the structure of described above, and Y is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

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Netphen, Germany

Norbert Kemnitzer