Boehringer Mannheim Gmbh

Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

Norbert Franken

Helmut Lenz

Gunter Pappert

Hans-Peter Josel

Ulrich Neumann

Hermann Leying

Guenter Ziegler

Nicholas R Hoyle

Beatus Ofenloch-Hahnle

Bernhard Eckert

Reiner Babiel

Judith Pinsl-Ober

Heinz Bodenmueller

Thomas Vess

Ellen Moessner

Apostolos Kyriatsoulis

Michael Rottmann

Method and apparatus, including computer program products, implement techniques for quantifying a target nucleic acid (target) in a test sample. The techniques include providing a target and a defined initial amount of an internal control nucleic acid (control) different from the target; amplifying the target and control in a common amplification process; measuring a quantity indicating the amount of amplification product for the target and for the control in relation to a parameter representing the progress of the amplification; determining a characteristic value of the progress parameter for the target based on measurement results related to the amount of target amplification product; possibly determining a characteristic value of the progress parameter for the control based on measurement results related to the amount of control amplification product; and quantifying the original amount of target according to a predefined or selected quantification scheme using at least the characteristic value for the target.

Quantification schemes for quantifying nucleic acids

The invention concerns calibrators or calibration solutions which are based on a human serum matrix and which are used in a method for detecting cytokeratin, a process for producing them, a method for stabilizing cytokeratin and an immunological method for detecting cytokeratin in a sample.

Stabilization of calibrators containing cytokeratin

In order to extend the measuring range and to reduce the Hook effect in an immunological method of determination of an antigen based on the principle of a sandwich assay it is preferable to use a method which is characterized in that the labelled detection molecule is an oligomer of a binding molecule selected from antibody or/and antibody fragment.

Method for reducing hook effect in an immunoassay

The present invention concerns a method for the determination of an analyte in a sample liquid using a metal complex capable of luminescence as an analyte-specific marker group for the production of a measuring signal in which an unspecific metal complex is additionally added as an interference elimination reagent which has a structure that is chemically related to the marker group.

Interference elimination reagent for the determination of an analyte

The present invention provides a storable POD conjugate solution, characterised by a pH value of 5.0 to 6.5, adjusted by means of an organic buffer substance with a concentration of 40 to 100 mmol/l, and 0.05 to 1.0 mol/l magnesium aspartate.

Storable protein solution

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Starnberg, Germany

Norbert Franken