Arizona State University

Arizona Board of Regents, a Body Corporate

Other

USA as Represented by Secretary of the Navy

Florida International University

Tf Health Corp.

Nongjian Tao

Erica S Forzani

Francis Tsow

Salah Boussaad

Shaopeng Wang

Dangdang Shao

Karan Syal

Di Wang

Yuting Yang

Rodrigo A Iglesias

Xiaojun Xian

Yan Guan

Chenwen Lin

Hui Yu

Di Zhao

Stuart Lindsay

Tianwei Jing

Wenlue Huang

Xiaonan Shan

Devon Bridgeman

Pengfei Zhang

Chenbin Liu

Wenwen Jing

Guanzhong Ma

Feimeng Zhou

Kyle James Foley

Alvaro Diaz Aguilar

Anant Rai

Yunze Yang

Rodrigo Iglesias

Detecting single molecules includes binding the single molecules to a surface of an optically transparent substrate, irradiating the surface of the substrate with light having an incident angle selected to achieve total reflection of the light, thereby scattering light from the surface and from the single molecules bound to the surface, and collecting light scatted by the surface and by the single molecules bound to the surface to form a series of images. Systems for detecting single molecules include an optically transparent substrate, a means for flowing a sample solution over a surface of the substrate, a light source configured to irradiate the surface, a camera, and a collection optical system configured to collect light scatted by the surface and by the target molecules on the surface to form a series of images on the camera.

Single molecule imaging

A stand-alone, fully integrated self-contained wearable metabolic analyzer for metabolic rate and respiratory quotient measurement. It includes a device body, disposable mask, and headgear. The device body comprises multiple different miniaturized modules, including a colorimetric sensing module, flow module, control circuit, power module, environmental sensors, wireless module, communication module, memory module, signal processing module, and display module. A disposable sensor chip, coated with chemical sensing probes, is utilized in the colorimetric sensing module for breath O2 and CO2 detection. A Venturi tube and pressure sensor-based flow module measures breath flow rate. The self-contained wearable metabolic analyzer derives physiological parameters including resting energy expenditure (REE), respiratory quotient (RQ), oxygen consumption (VO2), carbon dioxide production (VCO2), minute ventilation (VE), breath frequency (BF), and tidal volume (TV) from the measurement.

Self-contained wearable metabolic analyzer

A micro-colorimetric sensor for sensing target chemicals using edge tracking includes a substrate. A plurality of parallel linear channels of porous media is entrenched into the substrate and each linear channel includes a sensing material adapted to sense one of several specific target chemicals in air. The plurality of parallel linear channels is separated by barrier material from the adjacent parallel linear channel where the barrier material blocks diffusion of chemicals from one linear channel to another. A plate is affixed over the substrate top to cover the plurality of parallel linear channels. An air sample is diffused along the micro-colorimetric sensor and color images are captured. An intensity profile is derived from the plurality of color images to determine a maximum and a minimum intensity value along the sensor. A plurality of positions along the sensor is tracked to determine an edge position.

Method and apparatus for continuous gas monitoring using micro-colorimetric sensing and optical tracking of color spatial distribution

A method for deep learning video microscopy-based antimicrobial susceptibility testing of a bacterial strain in a sample by acquiring image sequences of individual bacterial cells of the bacterial strain in a subject sample before, during, and after exposure to each antibiotic at different concentrations. The image sequences are compressed into static images while preserving essential phenotypic features. Data representing the static images is input into a pre-trained deep learning (DL) model which generates output data; and antimicrobial susceptibility for the bacterial strain is determined from the output data.

Antimicrobial susceptibility testing with large-volume light scattering imaging and deep learning video microscopy

An apparatus for label-free analysis of molecules, including interactions and reactions of the molecules, is disclosed. The apparatus is based on detecting molecule movement under the influence of an external electric field. The apparatus is able to achieve sensitive detection of molecular binding to proteins or other molecules, and conformational changes of proteins or other molecules and biochemical reactions of the proteins or other molecules. Applications of the apparatus include screening of drug molecules, kinetic analysis of posttranslational modification of proteins, and small molecule-protein interactions.

Apparatus for analyzing and detecting interactions and reactions of molecules

A spirometry system includes an imaging device configured to capture upper body movement images of a subject during inhalation and exhalation of the subject. The system further includes at least one controller configured to receive the captured images from the imaging device and, based upon the received images, determine at least one of an image-based spirometry flow-volume curve for the subject or an image-based spirometry parameter for the subject.

Imaging-based spirometry systems and methods

An integrated method for assessing metabolic rate and maintaining indoor air quality and efficient ventilation energy use. A physical sensor assesses room occupancy. An actuated ventilation system is set to a constant COlevel in a predetermined healthy range, where the actuated ventilation system includes a COsensor. The actuated ventilation system sets a first air ventilation rate and the sensor measures a first COlevel. The system determines whether COlevel is in a healthy range, if not then the COlevel is adjusted by setting a subsequent air ventilation rate. A subsequent COlevel is measured. If the COlevel is determined to meet a predetermined healthy range, then an assessment of change of air ventilation rate (&#x394; ACH) is determined. The determination of air change rate can be further augmented by a physical pressure based measurement. The overall metabolic rate is generated.

Method and system for assessing metabolic rate and maintaining indoor air quality and efficient ventilation energy use with passive environmental sensors

A method for detecting biomarkers with shortened test time and maximized precision. A sample from the body fluid is made to flow over a sensor surface coated with a capture antibody to allow binding of a biomarker in the sample to the capture body. An optical method detects and counts the individual binding events along the sensor surface with single molecule resolution, and difference in the binding events along the sensor surface is detected in real time and analyzed to determine the biomarker concentration.

System and method for precision detection of biomarkers

A colorimetric sensor array includes a CMOS image sensor having a surface including pixels and a multiplicity of colorimetric sensing elements. Each sensing element has a sensing material disposed directly on one or more of the pixels. The colorimetric sensing elements are distributed randomly on the surface of the CMOS image sensor. Fabricating the colorimetric sensor array includes spraying a sensing fluid in the form of droplets directly on a surface of a CMOS image sensor and removing the solvent from the droplets to yield a multiplicity of sensing elements on the surface of the CMOS image sensor. Each droplet covers one or more pixels of the CMOS image sensor with the sensing fluid. The sensing fluid includes a solvent and a sensing material. The droplets are distributed randomly on the surface of the CMOS image sensor.

Chemical complementary metal-oxide semiconductor (CCMOS) colorimetric sensors for multiplex detection and analysis

A method for optical imaging of single protein molecules including tethering single protein molecules via a flexible polymer linker to a glass slide having a surface coated with an indium tin oxide (ITO) so that the single protein molecules are tethered to the coated surface. The single protein molecules are driven into oscillation by applying an alternating electric field to the coated surface and the glass slide is located in the field of view of an objective lens. Incident light is directed onto the coated surface from an angle to generate an evanescent field and produce scattered light. The scattered light is collected and imaged by a CMOS imager to record a sequence of images of the scattered light. A Fast Fourier Transform (FFT) filter is applied to each pixel of the recorded image sequence to produce an oscillation amplitude image from which size, charge, and mobility of the plurality of single protein molecules can be determined.

Optical imaging of single molecule size, charge, mobility, binding and conformational change

A system for identification of bacterial cells in free solution in a sample. A sample handler is adapted to position the sample. A light source illuminates a large volume of the sample. An imager is located to receive light scattered from the sample. A computer it is coupled to receive data transmitted from the imager. A controller is coupled to send control signals to the sample handler and the computer. The imager processes the scattered light to form images of the bacteria and transmits bacteria image information to the computer, wherein the bacteria image information includes intensity values and position data for the bacteria images from which the computer determines the presence of bacteria.

Fast bacteria detection and antibiotic susceptibility test by precision tracking of bacterial cells

A system and method for monitoring one or more physiological parameters of a subject under free-living conditions is provided. The system includes a camera configured to capture and record a video sequence including at least one image frame of at least one region of interest (ROI) of the subject's body. A computer in signal communication with the camera to receive signals transmitted by the camera representative of the video sequence includes a processor configured to process the signals associated with the video sequence recorded by the camera and a display configured to display data associated with the signals.

System and method for non-contact monitoring of physiological parameters

A micro-colorimetric sensor for sensing target chemicals that converts time sequence information into a spatial distribution of color. By tracking the spatial color distribution, chemical exposure over time is thus detected, which overcomes the limitation of traditional colorimetric sensors. A porous media is coated on a top surface of the substrate. Multiple sensing chemicals are fused in parallel linear channels into the porous media coating. A plate is affixed over the substrate top surface to cover the plurality of parallel linear channels. An air sample is diffused along the porous media to get a clear pattern of spatial color distribution and color images are captured. Optical parameters like gradient of spatial color distribution, intensity, and absorbance, etc., can be tracked to calculate analytes concentrations.

Provided herein are systems and methods for computer monitoring of remote photoplethysmography (rPPG) from camera images based on chromaticity in a converted color space, which reduces motion-induced artifacts in camera images for improved rPPG computer monitoring of physiological parameters. In particular, a rPPG system for monitoring at least one physiological parameter from image data is disclosed herein. A processor subsystem electronically receives a first image data set representative of a series of consecutive images of at least a portion of a living body. The processor subsystem converts the first image data set from a first color space to a second color space to generate a second image data set including first channel data comprising a luminance component and second channel data comprising a chromatic component. The processor subsystem processes the second channel data to monitor the at least one physiological parameter of the living body.

Systems and methods for computer monitoring of remote photoplethysmography based on chromaticity in a converted color space

A method for rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells including obtaining a biological sample from a subject including live bacteria. Different doses of antibiotic are added to a multi-well glass slide and adding portions of the biological sample to the wells. Bacterial cells are tethered onto the surface. The tethered bacterial cells are imaged and tracked. Bacterial sub-micron motion of tethered cells is measured at the different doses. A processor performs statistical analysis on a population of cells for each antibiotic dose to generate an antibiotic dose curve proportional to the motion changes, where the antibiotic dose curve plots data including a decrease in movement over time indicating a proportional effectiveness of an antibiotic applied to a well.

Growing community of inventors

Fountain Hills, AZ, United States of America

Nongjian Tao