Id Pharma Co., Ltd.

Dnavec Corporation

Noemi Fusaki

Mamoru Hasegawa

Hiroshi Ban

Yoshikazu Yonemitsu

Yasuji Ueda

Koichi Saeki

An objective of the present invention is to provide vectors for conveniently and efficiently producing ES-like cells in which foreign genes are not integrated into the chromosome. The present inventors discovered methods for producing ES-like cells from somatic cells using chromosomally non-integrating viral vectors. Since no foreign gene is integrated into the chromosome of the produced ES-like cells, they are advantageous in tests and research, and immunological rejection and ethical problems can be avoided in disease treatments.

Method for production of reprogrammed cell using chromosomally unintegrated virus vector

The present invention provides Sendai virus vectors in which genes that encode reprograming factors for inducing pluripotent stem cells are incorporated in a specific order, compositions comprising these vectors for gene delivery to be used in the induction of pluripotent stem cells, and uses thereof. Incorporation of the KLF gene, OCT gene, and SOX gene in a specific order into a single Sendai virus vector successfully and significantly increased the efficiency of pluripotent stem cell induction. Loading multiple reprogramming factors into a single vector can further increase the induction efficiency of pluripotent stem cells while reducing the number of necessary vectors.

Composition for inducing pluripotent stem cell, and use thereof

Growing community of inventors

Tsukuba, Japan

Noemi Fusaki