Dsm IP Assets B.v.

Noel Nicolaas Maria Elisabeth Van Peij

Martina Beishuizen

Jan Metske Van Der Laan

Alrik Pieter Los

Johannes Andries Roubos

Liang Wu

Lucie Parenicova

Peter Jozef Ida Van De Vondervoort

Robertus Antonius Mijndert Van Der Hoeven

Dick Schipper

Yvonne Johannes Odilia Arendsen

Marcus Hans

Olaf Leonardus Schouten

Jan Herman Pel

The present invention relates to a mutant microbial host cell which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide having &#x3b1;-amylase activity AmyC or an homologous thereof. It has been surprisingly found that when the mutant microbial host cell according to the invention is used in a method to produce a compound of interest, for example an enzyme, an improved purity of the product is obtained.

Amylase-deficient strain

The present invention relates to a method for carrying out recombination at a target locus.

Recombination system

The present invention relates to a method for improving protein yield. The method comprises modifying the value of a set of relevant protein features to fall within an optimal range or to become more close to an optimal value for one or more protein features in the eukaryotic host.

Method for improving the yield of a polypeptide

The present invention relates to a method for the production of a compound of interest by microbial fermentation, wherein the microbial host cell used has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The present invention further relates to a microbial host cell that has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The invention further relates to a compound of interest.

Recombinant host cell with deficiency in non-ribosomal peptide synthase production

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Echt, Netherlands

Noel Nicolaas Maria Elisabeth Van Peij