Dow Agrosciences LLC

Sangamo Therapeutics, Inc.

Agriculture Victoria Services Pty Limited

Dairy Australia Limited

Corteva Agriscience LLC

Noel Cogan

German Carlos Spangenberg

John White Forster

Manju Gupta

Steven Robert Webb

Matthew J Henry

Matthew Hayden

Tim Sawbridge

Jeffrey C Miller

Dmitry Y Guschin

W Michael Ainley

Yidong Ran

William M Ainley

John Mason

Hiroshi Shinozuka

Luke Pembleton

Nicola Patron

Sandeep Kumar

Stephen N Novak

Timothy Ivor Sawbridge

Kathryn Michaela Guthridge

Simone Jane Rochfort

Ben Cocks

Ben Hayes

W Mike Ainley

John Forster

John Gregory Mason

The present invention relates to methods for controlling hybridization in plants and producing hybrid plants. The present invention also relates to nucleic acids encoding amino acid sequences for self-incompatibility (SI) proteins in plants, and the use thereof for the manipulation of SI, including seed production, in plants, particularly of the Poaceae family. The present invention also relates to kits, compositions, constructs and vectors including such nucleic acids, and related polypeptides, regulatory elements and methods. The present invention also relates to expression of self-gamete recognition genes in plants and to related nucleic acids, constructs, molecular markers and methods.

Manipulation of self-incompatibility in plants

A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3&#x2032; ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Engineered transgene integration platform (ETIP) for gene targeting and trait stacking

The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organisms and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts selected from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont association.

Selection of symbiota by screening multiple host-symbiont associations

A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

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Macleod, Australia

Noel Cogan