Hitachi Plant Technologies, Ltd.

Hitachi, Ltd.

Hitachi Plant Services Co., Ltd.

Noe Miyashita

Koji Seo

Hideyuki Noda

Masahiro Okanojo

Shuichi Mori

Yuta Nakatsuka

Yoshiya Endo

Matsuo Kamitani

Ryusuke Gotoda

Harumasa Yamamoto

A microbe quantifying apparatus includes: a filtering mechanism that filters microbes contained in a fluid sample with a filter; a quantifying mechanism that quantifies the microbes with a specified biological material contained in the microbes on the filter as an index; and an anti-drying mechanism that prevents drying of the microbes on the filter.

Microbe quantifying apparatus and microbe quantifying method

Provided is a device for collecting biological material from a microbe, the device including: a collection container that is used to collect a microbe from a liquid sample containing the microbe and to extract the biological material from the microbe; and a temperature control mechanism that controls a temperature of the collection container. Herein, the temperature control mechanism controls a temperature of the collection container so that the microbe is collected at a first temperature and the biological material is extracted from the microbe at a second temperature higher than the first temperature.

Method for collecting biological material and device for collecting biological material

An object of the present invention is to provide an object-capturing device which accurately detects objects such as microorganisms captured at a test site. The object-capturing device of the present invention includes a capturing dish holding a carrier, which captures objects (microorganisms), on a first side of the capturing dish. The capturing dish has a through hole extending from the first side to a second side of the capturing dish. The object-capturing device is used in a way such that, after the objects are captured with the carrier directed upward, the carrier is directed downward and reagents for detecting the objects are contacted with the objects captured on the carrier through the through hole.

Device for capturing object and method for using the same

A collection unit ()includes: a collection carrier cartridge () formed, at its center, with a through hole () into which a nozzle for supplying hot water or ATP reagent is inserted including a carrier filling dish (), on an outer circumference of the through hole (), to be filled with a collection carrier () for collecting floating bacteria in the air, and an upper lid () on which the carrier filling dish () is placed, formed with a protrusion to be inserted through the through hole (); an impactor nozzle head () covering a surface of the collection carrier () and has a plurality of nozzle holes () facing the collection carrier () surface; and a fan () introducing air to the collection carrier surface through the nozzle holes (). A velocity of the air passing through the nozzle holes () is 40 m/s to 50 m/s.

Collection unit

The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.

Microbial detection apparatus, microbial detection method, and sample container used therein

Cartridge casing for sample collector

A reagent open mechanism of the luminescence measurement system comprises a triaxial actuator and a reagent dispensing nozzle which is driven by the triaxial actuator. A reagent cartridge where a reagent to be divided by the reagent dispensing nozzle is filled in a concave and the opening of the concave is sealed by an aluminum sheet can be set in. This reagent open mechanism comprises an open needle which is driven by the triaxial actuator and makes a hole in the aluminum sheet and a fixation block between the reagent dispensing nozzle and the open needle which arranges the reagent dispensing nozzle and the open needle in such location that the reagent dispensing nozzle or the open needle does not contact with a structure including the reagent cartridge in a Z-axis operation during opening time or reagent dividing and dispensing time.

Reagent open mechanism of luminescence measurement system and open needle control method in reagent open mechanism

The present invention provides for a reagent splitting/dispensing device and method that can prevent contamination of an operation fluid when a reagent dispensing nozzle is in a waiting state and prevent falling of a droplet. The reagent splitting/dispensing method includes disposing and adjusting an air layer between an interface of an operation fluid and a reagent aspirated and subsequently dispensed from a nozzle tip end in an reagent dispensing nozzle.

Growing community of inventors

Tokyo, Japan

Noe Miyashita