Kaneka Corporation

University of London

Nobuyoshi Umeda

Nobuhiko Sato

Shuhei Taniguchi

Hiroshi Sakurai

Takaaki Sakashita

Masahiro Hayashi

Keita Ino

Yuta Kita

Ken Suzuki

Chiho Kobayashi

Ayako Tsukamoto

An object of the present invention is to provide a cell population comprising safe adherent stem cells maintaining a normal karyotype and a method for producing the cell population, and a pharmaceutical composition comprising the cell population. According to the present invention, a production method of a cell population comprising adherent stem cells, comprising obtaining a cell population in which the proportion of KCNAB1-positive adherent stem cells in the cell population is 85% or more, is provided.

Cell population including adhesive stem cells, production method therefor and pharmaceutical composition

The present invention provides a multilayer matrix comprising at least two layers in which the first layer comprises a bioresorbable material and the second layer comprises a bioadhesive material, wherein the second layer contains cells. A method for producing a multilayer matrix and the use of a multilayer matrix are also provided.

Implantable cell dressing for treatment of disease

An object of the present invention is to provide highly proliferative mesenchymal stem cells (MSCs) useful for the large-scale and rapid production of a cell preparation, and a cell population comprising the mesenchymal stem cells. According to the present invention, there is provided a cell population comprising mesenchymal stem cells derived from the fetal appendage, wherein, in the cell population, the proportion of CD105mesenchymal stem cells is 50% or more, the proportion of CD200mesenchymal stem cells is less than 10%, and the proportion of CD106mesenchymal stem cells is less than 5%.

Cell population comprising mesenchymal stem cells derived from fetal appendage, method for producing the same, and pharmaceutical composition

Provided is a priming method including a step of filling a priming liquid in a hollow-fiber membrane module in which hollow-fiber membranes are packed in a vessel having an inlet port, an outlet port, and a filtrate discharge port at a linear velocity of 20 cm/min or more and 550 cm/min or less through the inlet port or the outlet port in an amount of 15% or more relative to a volume of the hollow-fiber membrane module. According to the present invention, the effective filtration area at the time of cell suspension treatment is increased, and the recovery rate of cells and the filtration rate can be improved. Further, since the cell treatment can be completed while maintaining the closed environment, the obtained cells can be provided for therapeutic applications.

Method for priming hollow-fiber membrane module

In a method for producing a cell concentrate using a cell suspension treatment system including a storage container of a cell suspension, which has a solution inlet port, a circulation outlet port, and a circulation inlet port, a cell suspension treatment device for concentrating the cell suspension by separating liquid from the cell suspension by filtration, the device including a container having a cell suspension introduction port, a cell suspension lead-out port, and a filtrate outlet, which is filled with a hollow fiber separation membrane, a circulation circuit for concentrating the cell suspension while circulating the cell suspension between the storage container and the cell suspension treatment device, a collection container of a cell concentrate obtained by concentration, a collection path for feeding the cell concentrate to the collection container, an injection path for injecting a solution into the solution inlet port of the storage container, and a detecting unit.

Method for producing cell concentrate, and cell suspension treatment system

The present invention has an object to solve the problem in a method for separating nucleated cells from a cell-containing fluid using a cell separation filter. Specifically, the present invention provides a cell separation filter and a method for preparing cells using a cell separation filter, which are capable of reducing unnecessary cell contamination in a nucleated cell fraction without needs of designing non-woven fabrics to suit cell species to be recovered. The present invention also provides a cell separation filter and a method for preparing cells using the filter capable of improving the recovery yield of nucleated cells. The present invention provides a cell separation filter comprising: a container having an inlet and an outlet, an adsorbent filled in the container, and a partition having an opening, the partition separating the adsorbent. The present invention also provides a method for preparing nucleated cells including the steps of: introducing a cell-containing fluid through the inlet of the cell separation filter to contact the cell-containing fluid with the adsorbent; and recovering a nucleated cell fraction from the filter.

Growing community of inventors

Settsu, Japan

Nobuyoshi Umeda