Mitsui Toatsu Chemicals, Incorporated

Mitsu Chemicals, Inc.

Nobuyoshi Makiguchi

Nobuhiro Fukuhara

Setsuo Yoshino

Yoshiyuki Nakajima

Maki Suzuki

Satori Sone

Nobumi Kusuhara

Shoichiro Miyahara

Atsunori Shindo

Kazunari Nitta

Masanobu Arita

Toshio Matsumoto

Kaoru Yamamoto

Shyoichiro Miyahara

Yoshiyuki Asai

Mitsuyoshi Nakamura

Mamoru Takeichi

Naoshi Hagihara

Kozo Sasa

Takeshi Nakamura

Shinichirou Tawaki

Ken-ichi Ishiwata

Tomoyuki Se

Hitoshi Tarukawa

Amino acid sequence of L-phenylalanine ammonia-lyase

A method for the production of human tissue type plasminogen activator (human t-PA) using cells is disclosed. The method comprises producing the human t-PA in parallel with the growth of the cells or after the cells have been grown. To the cell culture bicarbonate ion is added at a concentration equivalent to 3 to 10 g/l of NaHCO.sub.3 based on the true volume of the medium to increase the osmotic pressure of the medium to a range of 350 to 520 milliosmoles/liter.

Method for production of human tissue type plasminogen activator

A method for the production of human tissue type plasminogen activator (tPa) using cells is disclosed. The method includes a supplementation of a p-aminomethyl benzoic acid derivative to a cell culture medium or a tPA producing medium and further an increase of osmotic pressure in the medium to 350 milliosmoles or more/liter. The invention provides a method for producing single-chain tPA in a high concentration and with a relatively small amount of double-chain tPA in the medium.

Method for producing of human tissue type plasminogen activator

Escherichia coli carrying a hybrid plasmid having been constructed by inserting a desired foreign gene into an expression vector so as to permitting expression of said desired foreign gene therein was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coli (i.e., transformant) was cultured in the medium in which the sugar concentration was maintained at 0.3% or more in a first process so as to supress the suppression of the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression of the expression so as to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.

Method of regulating expression of a foreign gene by controlling the

The 5-substituted hydantoins represented below can be transformed into D-.alpha.-amino acids by the use of the cultured broth, cells or treated cells of the genus Hansenula: 5-substituted hydantoins: ##STR1## wherein R represents an alkyl, substituted alkyl, phenyl or substituted phenyl group. D-.alpha.-amino acids: ##STR2## wherein R represents the same meanings as above.

Process for producing D-.alpha.-amino acids

A recombinant plasmid for the expression of phenylalanine ammonia-lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus 10 region and (b) the P.sub.L promoter of the lambda phage, the tac promoter and the P.sub.L promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of in Escherichia coli.

Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase

Optically active serine can be racemized at a high reaction rate under the relatively mild conditions by treating the optically active serine in an alkaline solution in the presence of pyridoxal phosphate or salicylaldehyde and an alkaline compound.

Method for racemization of optically active serine

A luminous material includes a carrier and luminous bacteria dispersed in, admixed with, confined in or applied to the carrier, the material being useful in fishery, particularly as a fish bait.

Luminous material for use in fishery and method for the production

Growing community of inventors

Fujisawa, Japan

Nobuyoshi Makiguchi