National Institute of Advanced Industrial Science and Technology

Nobutaka Nakashima

Tomohiro Tamura

Yasuo Mitani

Provided is an expression vector capable of constitutive expression of a foreign gene in a bacterium belonging to the genus. The vector is a constitutive expression vector for a bacterium belonging to the genuscomprising: DNA comprising a nucleotide sequence of a mutated TipA gene promoter where a mutation is incorporated into a &#x2212;10 region sequence of a TipA gene promoter, the mutated TipA gene promoter being capable of thiostrepton-independent and constitutive expression of a gene located downstream thereof; a promoter sequence for the constitutive expression of a foreign gene; a ribosome-binding site sequence located downstream of the promoter sequence; and a multiple-cloning site sequence capable of incorporating a foreign gene therein, located downstream of the ribosome-binding site sequence.

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A microorganism of the genusis provided which has a higher sensitivity to lysozyme at a low concentration than a wild-type strain, which can easily cause cell lysis, and from which a recombinant protein expressed therein is easily recovered. More specifically, a mutant microorganism of the genushaving a higher sensitivity to lysozyme than a wild-type microorganism of the genus

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Hokkaido, Japan

Nobutaka Nakashima