Fuji Photo Film Company, Limited

Fujifilm Corporation

Nobuhito Masuda

Yuji Mihara

Masaki Okazaki

Yukio Yasuda

Shigeru Nagatomo

Nobuo Hiratsuka

Hajime Makiuchi

Yukio Sudo

Takushi Miyazako

Shigeki Kageyama

Takeshi Igarashi

Nobuo Suzuki

Tadashi Ikeda

Shigeo Hirano

Mitsunori Ono

Kentaro Nakamura

Shoji Ishiguro

Jun Arakawa

Yoshihiko Abe

Morihito Ikeda

Satoru Toda

Kazuya Kawasaki

Hiroko Inomata

Kenji Miura

Takushi, Both Miyazako

Yoshiro Kumano

It is an object of the present invention to provide a method capable of measuring a high concentration (up to 2500 U/L) of animal sample without dilutions, which is further capable of specifically measuring &#x3b1;-amylase even in a sample comprising animal &#x3b1;-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3). The present invention provides a method for specifically measuring &#x3b1;-amylase contained in a non-human animal sample comprising &#x3b1;-amylase and glucoamylase without diluting said sample, wherein measurement is carried out by using an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof, and pH of reaction is 6 or more, and less than 7.

Method for measuring animal &#x3b1;-amylase

An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived fromorsp. and cholesterol oxidase derived fromsp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.

Method for measuring high density lipoprotein cholesterol

The present invention provides a method for producing a dry analytical element for measurement of pancreatic lipase contained in a body fluid which contains triglyceride of long chain alkyl fatty acid having 12 to 22 carbon atoms, monoglyceride lipase, and a glycerine measurement reagent, and comprises a water-impermeable support and at least one spreading or reagent layer, said method comprising the step of coating an emulsion/dispersion solution of triglyceride with an average particle size of 1 &#x3bc;m or less.

Dry analytical element for lipase measurement

A dry-type analytical element for immunoassay utilizing antigen-antibody reaction in which the antigenic component is isolated from the antibody component by incorporating both components in separate layers or separately in a layer using different solvents. The analytical element is excellent in sensitivity and accuracy, and the analytical operation is simple.

Analytical element for immunoassay and method for its preparation

A synthetic substrate used for quantitative analysis of a trace enzyme. The substrate has a molecular structure (B)-(A)-(C) comprising at least one structure (A) catalytically affected by the enzyme to be analysed, at least one photographically active labelling structure (B) linked to the structure (A) and at least one specific adsorbing structure (C) linked to the structure (A). The substrate is used in a quantitative analysis of a trace enzyme, in which the structure (A) or the linkage between the structure (A) and the structure (C), or the linkage between the structure (B) and the structure (A) is cleaved by the action of the analyte enzyme. The reaction product of the enzymatic reaction is separated by allowing the same to contact with an adsorbent for the structure (C). The separated reaction product is developed photographically and then the optical density of the resultant developed silver and/or colored dye is measured.

Method for quantitative measurement of trace enzyme and substrate used

A method of preparing a dry-type analytical element for immunoassay having at least one water-permeable porous layer and containing a labeled antigen and an antibody to said labeled antigen and to an unlabeled antigen as the components participating in antigen-antibody reaction in said porous layer, which comprises applying or impregnating a first composition containing one of said labeled antigen and said antibody and thereafter applying or impregnating a second composition containing the other of said labeled antigen and said antibody dissolved or suspended in a solvent not dissolving substantially said labeled antigen or said antibody in the first composition onto said porous layer.

Method of preparing immunological analytical element

Modified proteins for carrying hapten are provided. These carriers are prepared by blocking the amino groups of the original protein and then introducing amino groups into the carboxyl groups of the original protein. The blocking groups may be eliminated at the later stage to regenerate the amino groups of the original protein. The modified protein or polypeptide carrier have the three-dimensional structures different from the original proteins so that they are used in immunoassay while carrying low molecular weight haptens without the fear of forming antibodies for the original proteins. The modified protein carrier may also be used in the passive agglutination immunoassay without the need of absorbing the anti-hapten antibodies by the hapten-carrying carriers.

Modified protein for carrying hapten

A multi-layered element for the quantative analysis of an immuno-reactant having a water-impermeable and light-transmissible support layer; a coloring reagent layer carried by said support layer and containing a hydrophilic polymer as a binder; and a reaction layer covering said reagent layer and made of a porous matrix. When the analyte immunoreactant is an antigen, said reaction layer contains the specific antibody for the antigen, the antibody being immobilized by a first water-insoluble carrier. When the analyte immuno-reactant is an antibody, said reaction layer contains the specific antigen for the antibody. The reaction layer contains an enzyme substrate immobilized by a second water-insoluble carrier different from said first water-insoluble carrier. The coloring reagent layer contains a detection reagent composition for coupling with an enzymatic reaction product produced by the reaction beween the enzyme-labelled complex of the immuno-reactant and the enzyme substrate immobilized by the second water-insoluble carrier. Upon coupling of the detection reagent composition with the enzymatic reaction product, a color is developed, the optical density of which is measured to determine the quantity of the analyte immuno-reactant contained in a liquid sample.

Multi-layered element for quantititative analysis of immuno reactant

A member of an analytical element for the analysis of a liquid sample containing solid material, comprising a volume filtration layer composed of a fibrous material and a spreading layer having a liquid-retaining capacity larger than that of the volume filtration layer, in which the volume filtration layer and the spreading layer are united to give an integrated structure. The spreading layer may be composed of a fibrous material, a woven cloth, a knitted cloth or a non-fibrous porous medium.

Member of analytical element for the analysis of liquid sample

A biological reaction layer of a dry analytical element in the form of a composite porous layer in which a particulate material carrying a biologically active material fixed thereon is dispersed in a fibrous porous matrix, characterized in that the dry weight ratio of said particulate material to said fibrous material contained in said composite porous layer ranges from 1:20 to 1:0.3 and said particulate material is contained therein in the range of 1 to 60 g/m.sup.2.

Biological reaction layer and its preparation

An instrument for separation of liquid from solid material comprising: a volume filtration layer having a void volume of not less than 90% which is composed of fibrous material; and a pressure-resistant housing having a liquid entrance and a liquid exit which encases said volume filtration layer therein in such a manner that the side face of the volume filtration layer is tightly arranged in contact with an inner face of the housing so as to prevent passage of the solid material along the interface between the side face of the volume filtration layer and the inner face of the housing.

Process and apparatus for removing solid material including blood cells

An integral element for biological reaction comprising at least two layers consisting essentially of:

Integral element for biological reaction and process for the preparation

A dry type multilayer analysis element for assaying a concentration of a specific component utilizing a competitive immunological reaction comprises a detection element comprising a detection layer which receives a labelled complex formed as a result of the competitive immunological reaction or an optically detectable change formed dependent upon an amount of the labelled complex of the specific component and having further provided thereon the detection layer a reaction layer comprising a fibrous porous medium containing fine particles therein. The multilayer analysis element absorbs an amount of a sample solution necessary for the competitive immunological reaction so that the multilayer analysis element has high sensitivity and high reproducibility.

Multi-layer analysis element utilizing specific binding reaction

A method for immunochemical assay of an antigen or antibody by labelling the antigen or antibody with a specific cyanine or merocyanine dye containing a carboxy group followed by effecting an immune reaction and photochemical processing thereof is provided. The amount of the antigen or antibody is measured in term of optical density of developed silver halide which is brought into contact with either the antigen-antibody reaction product or the unreacted material. This immunochemical assay method gives high detection sensitivity in a simple operation manner.

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Asaka, Japan

Nobuhito Masuda