Toyota Jidosha Kabushiki Kaisha

Kabushiki Kaisha Toyota Chuo Kenkyusho

Toyota Jidosha Kabuhsiki Kaisha

Kabuhsiki Kaisha Toyota Chuo Jenjyusho

Nobuhiro Ishida

Kenro Tokuhiro

Toru Onishi

Nobuki Tada

Satoshi Katahira

Noriko Yasutani

Haruo Takahashi

Risa Nagura

Chie Imamura

Masana Hirai

Satoshi Saitoh

Eiji Nagamori

Tohru Ohnishi

Takaaki Hatanaka

Akihiko Kondo

Takao Tani

Takao Imaeda

Takashi Shimamura

Hibiki Matsushita

Osamu Saotome

Chiaki Ogino

Kazuhide Tabata

Risa Nakamura

Satoshi Saito

Kazunori Nakashima

Yasuo Matsuo

Chikara Miyazaki

Sung-Jin Jo

A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.

Protein having xylose isomerase activity and use of same

A method for identifying and separating a rare earth ion. The method includes bringing one or more rare earth ions into contact with a mineralization agent capable of mineralizing the rare earth ions, wherein the one or more rare earth ions are separated as rare earth minerals, based on a mineralization tendency of the mineralization agent with respect to a rare earth ion series.

Method for separation of rare earth ion

A binding agent is capable of binding to rare earth materials such as rare earths and inorganic compounds thereof. A rare earth material-binding agent includes a peptide capable of binding to a rare earth material including a rare earth and a rare earth inorganic compound.

Rare earth material-binding peptide and use thereof

The invention is intended to improve xylose assimilation ability and ethanol fermentation ability in a xylose-assimilating yeast into which a xylose isomerase gene has been introduced. The amount of NADH produced by the recombinant yeast into which the xylose isomerase gene had been introduced as a result of the enzymatic reaction of acetohydroxy acid reductoisomerase is lowered.

Recombinant yeast and method for producing ethanol using the same

The present invention provides a method for producing a useful substance efficiently from cellulose without using any cellulase preparation. According to the disclosures of the present specification, in the production of a useful substance from a cellulose-containing material, the cellulose-containing material is brought into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material, and a carbon source comprising the cellulose in the cellulose-containing material is simultaneously saccharified and fermented by a cellulase-producing microorganism in the presence of the ionic liquid.

Method for producing useful substance from cellulose-containing material

The present invention provides, inter alia, an improvement in the production of a desired product from yeast, wherein the growth rate and fermentation rate of the yeast are maintained at excellent levels. The present invention also provides, inter alia, for the introduction of a foreign gene into a yeast host cell, wherein the foreign gene encodes an enzyme involved in the production of a desired product. The yeast may also constitutively express a HAP4 gene, or a homologous gene thereof, to significantly improve the ability of the yeast to produce the desired product while maintaining its growth rate and fermentation rate. In some embodiments, a yeast mutant is used which is a mutant strain having lowered alcohol productivity vis-&#xe0;-vis a wild-type strain.

Yeast mutant and substance production method using the same

The present invention provides a transformant into which has been incorporated DNA for coding a foreign protein having lactate dehydrogenase activity and provided with pyruvic acid substrate affinity that equals or exceeds the pyruvic acid substrate affinity of the pyruvate decarboxylase inherent in the host organism. Said transformant can stably mass-produce lactic acid inside a host organism having the pyruvate decarboxylase gene.

Method of controlling ethanol production and mass production of lactic acid and transformant therefor

This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).

DNA encoding a protein having D-lactate dehydrogenase activity and uses thereof

The invention provides a promoter that can be used in the presence of an organic acid. The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts.

Promoter in the presence of organic acid and utilization thereof

Growing community of inventors

Seto, Japan

Nobuhiro Ishida