Ajinomoto Co., Ltd.

Nobuharu Tsujimoto

Hisashi Yasueda

Shinichi Sugimoto

Yoshio Kawahara

Kazuhiko Matsui

Hisao Ito

Tomoko Suzuki

Osamu Kurahashi

Eiji Ono

Hiroshi Matsui

Yoshihiro Usuda

Tsuyoshi Nakamatsu

Hiroshi Izui

Eiichiro Kimura

Yoko Asakura

Yoshiya Gunji

Megumi Shimaoka

Hiroko Kishino

Chizu Abe

Yuri Miyata

Manami Oba

Kumiko Fukase

Ken-ichi Hashiguchi

A method for producing a target substance by utilizing a microorganism by culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture is described. The microorganism is a mutant recombinant strain in which maltose assimilation is controlled by reducing or eliminating the interaction between IIAprotein of the glucose PTS and a protein involved in non-PTS uptake of maltose.

Method for producing target substance by fermentation

An L-amino acid is produced by culturing abacterium which can grow by using methanol as a main carbon source and has L-amino acid-producing ability, for example, abacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation through introduction into cells, of a DNA coding for dihydrodipicolinate synthase that does not suffer feedback inhibition by L-lysine and a DNA coding for aspartokinase that does not suffer feedback inhibition by L-lysine, or abacterium made to be casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.

[object Object]

Anmutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library ofand a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase (named dapB) is obtained from the transformant.

The present invention describes a method for producing a target substance by utilizing a microorganism comprising culturing the microorganism in a medium, allowing the target substance to accumulate, and collecting the target substance from the medium. Also the microorganism used in the present invention is a mutant strain whereby maltose assimilation is controlled by the interaction between IIAprotein of glucose PTS and MalK.

Anmutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed by using a chromosome gene library ofa transformant strain which can grow on a minimal medium is selected, and recombinant DNA containing DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant.

Gene encoding dihydrodipicolinate synthase from Bacillus methanolicus and methods of making lysine wing said gene

The present invention provides dihydropicolinate synthase and dihydrodipicolinate reductase enzymes from polynucleotides encoding the enzymes, and methods of producing L-lysinse in microorganisms expressing the enzymes.

Dihydrodipicolinate synthase from Bacillus methanolicus

Process for producing L-glutamic acid by fermentation

An L-isoleucine-producing bacterium belonging to the genus Escherichia which carries a thrABC operon which comprises a thrA gene coding for aspartokinase I-homoserine dehydrogenase I substantially released from the inhibition by L-threonine and an ilvGMEDA operon which comprises an ilvA gene coding for threonine deaminase substantially released from the inhibition by L-isoleucine and whose region required for attenuation is removed; and an L-isoleucine-producing bacterium belonging to the genus Escherichia carrying the thrABC operon, an lysC gene coding for aspartokinase III substantially released from the inhibition by L-lysine, and the ilvGMEDA operon. The bacteria permit biosynthesis of a sufficient amount of L-isoleucine.

L-isoleucine-producing bacterium and method for preparing L-isoleucine

.alpha.-Ketoglutarate dehydrogenase gene

A fermentative process for producing L-glutamic acid efficiently and at low cost is disclosed. Also disclosed are microorganisms having improved ability to produce L-glutamic acid. These microorganisms belong to the genus Escherichia, have resistance to an aspartic acid antimetabolite, and are deficient in .alpha.-ketoglutaric acid dehydrogenase activity. The process comprises cultivating one of these microorganisms in a liquid medium, accumulating L-glutamic acid in the culture medium, and collecting L-glutamic acid. In particular, E. coli AF13199 (FERM BP-5807).

Process for producing L-glutamic acid by fermentation with E. coli

A mutant of the genus Escherichia is described, the .alpha.-ketoglutarate dehydrogenase activity of which is deficient or reduced, and/or the phosphoenol pyruvate carboxylase and/or glutamate dehydrogenase activities of which are amplified. The mutant is useful in the fermentative production of L-glutamic acid.

Growing community of inventors

Kawasaki, Japan

Nobuharu Tsujimoto