Ventria Bioscience

Ventria Biosciences Inc.

Applied Phytologics, Inc.

Ning Huang

Raymond L Rodriguez

Frank E Hagie

David M Stalker

Mary Ann Michelle Fernandez Santos

Steven Clyde Pettit

Daichang Yang

Yong-Sic Hwang

Robert J Schmidt

Diane Phuong Nguyen

Paula Bryan

Raymond L Rodriquez

Jianmin Huang

Delia R Bethell

Deshui Zhang

Scott Deeter

Joseph E Schmidt

Kenneth J Mabery

The present invention relates to cell culture media supplements or complete media compositions comprising plant-produced heterologous recombinant human albumin, as well as methods of making the cell culture media, and methods of using the supplemented cell culture media to improve viability, productivity, and growth characteristics of cultured cells.

Cell culture media containing combinations of proteins

The present invention relates to cell culture media supplemented with a plant-produced recombinant mammalian albumin supplement, as well as methods of making the cell culture media, and methods of using the supplemented cell culture media to improve growth characteristics of cultured cells.

The present invention relates to cell culture media containing combinations of proteins, as well as methods of making the cell culture media, and methods of using the cell culture media to improve growth characteristics of cultured cells.

Disclosed are compositions and methods of making non-glycosylated transferrin protein in transgenic monocot plants.

Non-glycosylated transferrin expressed in monocots

The invention is directed to blood proteins produced in monocot seeds and isolated therefrom for use in therapeutic compositions, as well as to methods of making these isolated blood proteins and to therapeutic compositions comprising them.

Monocot seed product comprising a human blood protein

The invention is directed to monocot plant seed products comprising human serum albumin, and to methods of making said monocot plant seed products comprising human serum albumin and to therapeutic compositions comprising them.

Monocot seed product comprising a human serum albumin protein

The invention is directed to seed and seed extract compositions containing levels of a human milk protein between 3-40% or higher of the total protein weight of the soluble protein extractable from the seed. Also disclosed is a method of producing the seed with high levels of extractable human milk protein. The method includes transforming a monocotyledonous plant with a chimeric gene having a protein-coding sequence encoding a protein normally present in human milk under the control of a seed maturation-specific promoter. The method may further includes a leader DNA sequence encoding a monocot seed-specific transit sequence capable to target a linked milk protein to a storage body.

Expression of human milk proteins in transgenic plants

The invention relates to transgenic plants that demonstrate enhanced expression of a plant transcription factor under the control of a seed specific promoter such that expression of the transcription factor activates transcription of a native or non-native coding sequence in the plant. The invention further relates to a method of generating such transgenic plants and methods for enhancing the level of expression of a selected heterologous protein in seeds of such transgenic plants.

Plant transcription factors and enhanced gene expression

A method of producing human serum albumin (HSA) in monocot plant seeds, by transforming a monocot plant cell with a chimeric gene containing a promoter from the gene of a maturation-specific monocot plant storage protein, a first DNA sequence operably linked to the promoter and encoding a signal sequence, and a second DNA sequence linked in translation frame with the first DNA sequence and encoding HSA, where the first DNA sequence and the second DNA sequence together encode a fusion protein containing an N-terminal signal sequence and the HSA; growing a monocot plant from the transformed monocot plant cell for a time sufficient to produce seeds containing the HSA; and harvesting the seeds from the plant.

Expression of human serum albumin (HSA) in monocot seeds

This invention concerns a method of making an anti-infective composition for oral administration using an agent selected from the group consisting of lactoferrin protein and lysozyme protein. The method comprises three steps. The nucleic acid, which encodes a lactoferrin or lysozyme protein, is expressed in a monocot seed. The protein is isolated from the seed. This protein isolation compromises greater than 3% of the total soluble protein in the monocot seed. The protein is then formulated for oral administration.

Method of making an anti-infective composition for treating oral infections

The invention is directed to food and food additive compositions comprising one or more human milk proteins produced in the seeds of a transgenic plant and methods of making the same. The invention is further directed to improved infant formula comprising such food supplement composition.

A plant transformation vector for transforming host plant cells with a chimeric selectable marker gene is disclosed. The gene includes, operatively linked in sequence in a 5&prime; to 3&prime; direction, (i) a DNA promoter sequence from the rice beta-glucanase 9 (gns9) gene; (ii) a selectable marker gene, and (iii) a 3&prime; untranslated terminator region. Also disclosed are a vector pair containing the transformation vector, a method of obtaining transformed monocots whose seeds produce a selected heterologous protein during sed germination, and a plant whose cells are transformed with the chimeric selectable marker gene.

Plant selectable marker and plant transformation method

A plant transformation expression cassette for transforming host plant cells with a selectable marker gene is disclosed. The cassette includes, operatively linked in sequence in a 5&prime; to 3&prime; direction, (i) a DNA promoter sequence from the rice beta-glucanase 9 (gns9) gene; (ii) a selectable marker gene, and (iii) a 3&prime; untranslated terminator region. Also disclosed are a expression cassette pair containing the transformation vector, a method of obtaining transformed monocots whose seeds produce a selected heterologous protein during sed germination, and a plant whose cells are transformed with the chimeric selectable marker gene.

Growing community of inventors

Davis, CA, United States of America

Ning Huang