Teknologian Tutkimuskeskus Vtt Oy

Danisco US Inc.

Vtt Technical Research Centre of Finland Ltd

Nina Aro

Tiina Pakula

Kaisa Marjamaa

Michael C Ward

Markku Saloheimo

Kristiina Kruus

Anu Koivula

Mari J Valkonen

Felipe Oseas Bendezu

Jenni Rahikainen

Yun Luo

The present invention concerns a process for the dissolution of cellulose using an enzymatic treatment, followed by a chemical dissolution. Particularly, the invention relates to the utilization of oxidizing enzymes for said enzyme treatment step. The process of the invention will cause an improved dissolution of high molar mass cellulose fibres. The improved dissolution properties also cause an improved reactivity of the cellulose in subsequent reactions.

Fibre dissolution with enzymatic treatment

The present invention discloses a polypeptide having beta-glucosidase activity. The activity is retained also in high glucose concentration. The invention also discloses an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing the polypeptide. Further the invention discloses compositions including the polypeptide and method of using the polypeptide in hydrolysis or synthesis.

Beta glucosidase with high glucose tolerance, high thermal stability and broad PH activity spectrum

Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.

Protein production in filamentous fungal cells in the absence of inducing substrates

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Vtt, Finland

Nina Aro