Kws Saat Se * Co. Kgaa

Nils Stein

Milena Ouzunova

Britta Schulz

Monika Kloiber-Maitz

Christof Bolduan

Markus Niessen

Frank Breuer

Silke Wieckhorst

Jutta Menzel

Takayoshi Ishii

Peer Wilde

Ruonan Zhou

Jochen Kumlehm

Bernd Hackauf

Viktor Korzun

Andreas Houben

Raheleh Karimi-Ashiyani

The present invention relates to non-transgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the α2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.

Generation of haploid plants

Hybrid cereals are described which are obtained by restoring the pollen fertility for the Pampa cytoplasmic male sterility (P-CMS) and which are characterized by a reduced linkage drag. Plants are provided, in particular rye, which, as the male pollen parent, are capable of restoring the pollen fertility for the P-CMS. Furthermore, the nucleic acid molecule which carries the necessary information for restoring the P-CMS, DNA and vectors which contain such a nucleic acid molecule, corresponding host cells as well as a protein which can be encoded by the nucleic acid molecule and antibodies directed against it are also described. Furthermore, methods for the production of corresponding hybrid plants and transgenic plants are provided.

Restorer plants

The present invention relates to non-tr&#xe4;nsgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the &#x3b1;2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.

Growing community of inventors

Munich, Georgia

Nils Stein