Cytiva Sweden Ab

Ge Healthcare Bio-sciences Ab

Nils Stafstrom

Bjorn A Johansson

Lennart Bjorkesten

Andreas Marcstrom

Gunnar Malmquist

Gustav Jose Rodrigo

Martin Hall

Enrique Napoleon Carredano

Tomas Yngve Agren

Tim Francois

Kerstin Elisabet Erickson

Lars Olof Nils Rosengren

Yngve L Hareland

Anna Andersson

Anita Jonsson

Jeanne Cheng

The present invention relates to a nozzle for an air trapping device configured to remove air from a fluid, the nozzle comprising a body having an input opening configured to receive the fluid and an output opening configured to distribute the fluid along an edge of the output opening, wherein the edge comprises a control element configured to reduce surface tension of the fluid. The present invention further relates to an air trapping device configured to remove air from a fluid and comprising the nozzle.

Air trapping device and nozzle therefore

A method for fraction collection and a fraction collection system () comprising: —a dispensing device () including an outlet () configured for dispensing fractions of a liquid received from a connected system; —plural receptacles () configured for receiving one or more of said fractions, wherein the receptacles () and the dispensing device () are movable in relation to each other into a number of different positions such that the dispensing device () is capable of dispensing a respective fraction of said liquid into one or more of the receptacles (); —a waste collection device (), which is arranged in the fraction collection system () such that it is movable into at least a first and a second position wherein, in said first position, an inlet () of the waste collection device () is positioned below the outlet () of the dispensing device () such that liquid being dispensed from the outlet () of the dispensing device () is received in the waste collection device () and wherein, in the second position, the inlet () of the waste collection device () is not positioned below the outlet () of the dispensing device () such that fractions being dispensed from the dispensing device () can be received in one or more of the receptacles ().

Fraction collection system and method

Disclosed is a method for determining an operating flow rate for a chromatographic column () in an HPLC system (). The method comprises: measuring/calculating a pressure of the system () without the chromatographic column () for one or more flow rates; fitting a function to the flow rate(s) and corresponding pressure(s), calculating from the function and a predetermined recommended flow rate for the chromatographic column () a system pressure drop at the predetermined recommended flow rate. An operating flow rate is determined by summing the system pressure drop and a maximal column pressure limit, and determining a contribution of the system pressure drop to the summed pressure. If this contribution exceeds 1% an operating flow rate for the column is determined to a flow rate that corresponds to a pressure at a pressure monitor arranged before the column that is lower than the predetermined maximum column pressure limit.

Method for determining an operating flow rate for a chromatographic column in an HPLC system

Disclosed is a chromatography system () comprising: plural modules (-) including at least one pump and a column valve unit () connectable to plural chromatography columns; and a controller (), the controller being operable to control the or each pump and the column valve to perform different chromatographic processes, including chromatography employing just one column, as well as chromatography employing two or more columns by selective valve opening in said unit. The system includes a housing () into which the plural modules (-) are interchangeably mountable in apertures of one generally vertical face of housing, the modules are adapted for selective fluidic interconnection by tubing substantially at said one face such that in use the modules and tubing occupy a generally vertically extending volume to minimize the footprint of the system.

Chromatography apparatus

The present invention relates to a nozzle for an air trapping device configured to remove air from a fluid, the nozzle comprising a body having an input opening configured to receive the fluid and an output opening configured to distribute the fluid along an edge of the output opening, wherein the edge comprises a control element configured to reduce surface tension of the fluid. The present invention further relates to an air trapping deviceconfigured to remove air from a fluid and comprising the nozzle.

The present invention relates to a method for determining size of biomolecules separated in a medium by an electric field using marker molecules of known size, comprising &#x2014;() detecting a plurality of bands and forming a detected marker sequence and a detected unknown sequence based on a separation criterion, &#x2014;() determining band properties for each detected band, &#x2014;() comparing the band properties of the detected bands of the detected marker sequence with known band properties for a plurality of marker molecules forming a known marker sequence and assigning a score to each comparison, said score being based on at least one of relative distance, relative intensity, expected distance and expected intensity between bands, &#x2014;() selecting the comparison with the highest score and associating all or a subset of the detected bands of the detected marker sequence with said plurality of marker molecules of the known marker sequence in accordance with said comparison to determine size of the all or a subset of the detected marker sequence, and &#x2014;() comparing the bands of the detected marker sequence with the bands of the detected unknown sequence to determine a size of biomolecules for each identified band of the detected unknown sequence based on the known sizes of the marker molecules. The invention also relates to software configured to perform the method and to a computer readable medium for storing said software.

Method for determining a size of biomolecules

The invention discloses a method for correcting a baseline in a chromatogram obtained using a buffer with at least one UV-absorbing component which is a weak acid or a weak base. The method comprises the steps of: a) providing a chromatography system with a chromatography column and a UV detector downstream of the chromatography column; b) providing UV extinction coefficients at a wavelength &#x3bb; for the acid form and the base form of the UV-absorbing component(s) and a dissociation constant for the component(s), c) obtaining a chromatogram with the UV detector at wavelength &#x3bb; using the buffer under a set of conditions where pH and/or conductivity is varied between these conditions, d) for a plurality of said conditions calculating the concentrations of the acid and base forms using the dissociation constant(s) and values for pH and conductivity values, and e) calculating UV absorption values of the acid and base forms from the concentrations and UV extinction coefficients under the conditions in question and subtracting the UV absorption values from the chromatogram.

Method for baseline correction in a chromatogram

A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-H&#xfc;ckel, wherein the ion size parameter a in the Debye-H&#xfc;ckel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.

Growing community of inventors

Uppsala, Sweden

Nils Stafstrom