Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Cytiva Sweden Ab

Cytiva Bioprocess R&d Ab

Nils Norrman

Gunnar Glad

Bo-Lennart Johansson

Jean-Luc Maloisel

Andreas Axen

Anders Larsson

Ronnie Palmgren

Tobias Soderman

Jan Bergstrom

Bjorn A Johansson

Henrik Ostlin

Stevan Paul Tortorella

David Gwyn Treharne

Ola Lind

Henrik Ihre

Stefan D'Argy

Ulrika Meyer

Susanna Lindman

Klaus Gebauer

Gustav Jose Rodrigo

Sven Wounder

Eric Faldt

Mathias Alterman

Mattias Algotsson

Andreas Torbjorn Lundin

Peter Mats Oliviusson

Christer Olof Eriksson

Johan William Ohman

Andreas Axén

Johan Fredrik Avallin

Dag Lindstrom

Therese Graner

Karl Axel Jakob Liderfelt

David Carl Martin Bergman

Pierre Le Greves

Karl Liderfelt

Ake Stenholm

Henrik Östlin

Jesper Hedberg

Niklas Jungnelius

Anita Solbrand

Johan Avallin

Stefan D Eriksson

Tobias Ingemar Solderman

Asa Rudstedt

Tobias Ingemar Soderman

The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.

Magnetic immunoglobulin-binding particles

A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: &#x2014;providing a feed from a cell culture () comprising said biomolecule to a magnetic separator () and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; &#x2014;separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; &#x2014;forwarding said magnetic beads as a slurry with an added buffer to an elution cell (); &#x2014;eluting the bound biomolecules in the elution cell.

Method and system for separating biomolecules

The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.

Chromatography medium

The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.

Method and chromatography medium

The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.

Separation matrix

Automated two step chromatography purification system comprising a, system controller, a capture flow path comprising at least one pump, an elution flow path comprising at least one pump, and a valve arrangement for selective connection of two capture columns to the capture flow path and the elution flow path respectively such that both flow paths may be operated simultaneously and in parallel.

Automated multi-step purification system

The present invention provides a microplate comprised of multiwell strips connected in such a way as to permit the microplate to unfold and to pass in a linear fashion past a fraction collector and then re-fold back into a microplate configuration. Furthermore, the invention provides for the use of the microplates of the invention in a variety of different laboratory methods.

Foldable microplate

A method for purifying a substance in a solution in a simple streamlined process using a magnetic porous particle. For easy small scale purification of a substance, the magnetic porous particle is coated with either a hydrophilic or hydrophobic liquid and transferred into a second liquid containing the substance under conditions which allow said substance to partition into the first liquid within said magnetic porous particle. Finally the magnetic porous particle is removed from said second liquid, wherein the first and second liquid are substantially immiscible and the partition coefficient P of the substance between the first and second liquid is greater than 1.

Extraction separation using magnetic beads

The present invention provides methods and systems for eluting and an analyte from a solid phase. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a solid phase to a second vessel, such as a microtiter plate well. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.

Circular extractor

The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.

Cross-linked cellulose membranes

The present invention relates to a novel non-cross linked chromatography media provided with glutathione ligands which may or may not be provided with magnetic particles. The chromatography media is used for production of an affinity media provided with gluthatione ligands for adsorption of GST-tagged proteins.

Chromatography media and use thereof

The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.

Separation medium for chromatography of various biomolecules

The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

Manufacture of chromatography matrices

The present invention relates to a device and method for separation of proteins and other biomolecules. A preferred use is for sample preparation of crude as well as pre-fractionated samples. In a preferred embodiment, the device of the present invention is a pipette tip having dual channels, one for inlet of sample and one for outlet. The outlet, but not the inlet, channel is provided with sample separation media for separation of a desired biomolecule from a sample. The flow through the sample separation media is unidirectional.

Device and method for separation of proteins and other biomolecules

Growing community of inventors

Uppsala, Sweden

Nils Norrman