Zumutor Biologics Inc.

Nikitha M

Vivek Halan

Kavitha Iyer Rodrigues

Sunit Maity

Sanghamitra Bhattacharjee

Maloy Ghosh

Bharath Ravindra Shenoy

Sahana Bhima Rao

Divya Unnikrishnan

Anuradha Hora

Karthika Nair

Sohang Chatterjee

Aswini Thanigaivel

Bhargav Prasad

Sankaranarayanan Srinivasan

Subhra Prakash Chakrabarty

Yogendra Manjunath Bangalore Muniraju

Bairavabalakumar Natarajan

Sathyabalan Murugesan

Amol Maliwalave

Ashvini Kumar Dubey

Anurag Tiwari

Santosh Kumar

Veeresha Kamanagowda

Amir Khan

Pravin Kumar Dakshinamurthy

Pavithra Mukunda

Shivani Patel

Ankurina Sharma

Rashmi Sharma

Yogendra Manjunath Muniraju

The present disclosure relates to vectors for cloning and expressing genetic material including but not limiting to antibody gene or parts thereof and methods of generating said vectors. Said vectors express the antibody genes in different formats such as Fab or scFv as a part of intertransfer system, intratransfer system or direct cloning and expression in individual display systems. In particular, phage display technology is used to clone and screen potential antibody genes in phagemid which is followed by the transfer of said genes to yeast vector for further screening and identification of lead molecules against antigens. The present vectors have numerous advantages including uniquely designed inserts/expression cassettes resulting in efficient and smooth transfer of clonal population from phage to yeast vectors resulting in efficient library preparation and identification of lead molecules.

Vectors for cloning and expression of proteins, methods and applications thereof

The present disclosure relates to a method of generating an antibody library, not limiting to a human na&#xef;ve antibody gene expression library encompassing a pool of nucleic acid sequences derived from a natural antibody repertoire comprising humoral immunity from healthy and genetically diverse human populations. More specifically, the method employs a combination of phage and/or yeast antibody surface display concept which allows to screen large antibody library size and facilitates better folding of antibody structure. The present disclosure also relates to a human na&#xef;ve antibody library generated by employing the process of the present disclosure, a set of primers employed in the method and application(s) of said antibody library.

Method of generating an antibody na&#xef;ve library, said library and application(s) thereof

Growing community of inventors

Bengaluru, India

Nikitha M