Applied Biosystems, LLC

Nigel P Beard

Charles Stuart Vann

James C Nurse

Timothy G Geiser

Debjyoti Banerjee

Mark F Oldham

Joy Roy

Dennis Lehto

Ian A Harding

Yuh-Min Chiang

Umberto Ulmanella

David M Liu

Douglas Greiner

Alexander A Dromaretsky

Joon Mo Yang

Sergey V Ermakov

Maryam Shariati

John R Van Camp

Min Yue

Kenneth J Livak

Dar Bahatt

Kevin S Bodner

Stephen J Gunstream

H Pin Kao

Jason E Babcoke

A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.

Electrowetting dispensing devices and related methods

Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.

Systems and methods for multiple analyte detection

A device having a first substrate and a second substrate separated from the first substrate to form a volume between the first and second substrates, electrodes disposed on the first and second substrates and facing the volume, each substrate comprising at least one electrode, and a first insulating layer disposed on the first substrate and a second insulating layer disposed on the second substrate to separate the electrodes from the volume.

Exemplary embodiments provide microfludic devices and methods for their use. The microfluidic device can include an array of M&#xd7;N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.

Microfluidic devices and method for their use

A device and methods for sample distribution through a channel in which an expandable valve provides a mechanism to regulate flow through the channel. The valve may be configured to exert a force on a membrane layer so as to substantially block a portion of the channel to retain the sample in a desired location and prevent flow past the valve mechanism between the channel and a chamber.

Sample distribution devices and methods

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San Diego, CA, United States of America

Nigel P Beard