Abbott Laboratories Corporation

Abbott Diabetes Care Inc.

Nigel John Forrow

Gurdial Sanghera

Jared L Watkin

Xiang Cheng Zhang

Catherine Ann McTigue

Shridhara Alva Karinka

Stephen Walters

Stephan Walters

Jared Watkin

Simon William Bayliff

Simon W Bayliff

Gurdial S Sanghera

The claimed subject matter relates to the stabilization of 1,2-quinone mediators, especially those containing 1,10-phenanthroline quinone (PQ) and more especially transition metal complexes of PQ, in the presence of enzymes when contained in dry reagent layers for biosensor electrodes, through the use of various metal salts, particularly those of lithium.

Mediator-stabilized reagent compositions for use in biosensor electrodes

Mediator stabilized reagent compositions for use in biosensor electrodes

A biosensor for determining the concentration of an analyte in a biological sample. The biosensor comprises a support, a reference electrode or a counter electrode or both disposed on the support, a working electrode disposed on the support, the working electrode spaced apart from the other electrode or electrodes on the support, a covering layer defining a sample chamber over the electrodes, an aperture in the covering layer for receiving a sample, and at least one layer of mesh in the sample chamber between the covering layer and the electrodes. The at least one layer of mesh has coated thereon a silicone surfactant. Certain silicone surfactants are as effective as fluorinated surfactants with respect to performance of biosensors. These surfactants, when coated onto the mesh layer of the biosensor, are effective in facilitating the transport of aqueous test samples, such as blood, in the sample chamber.

Biosensor

The present invention is based on the discovery of NADand NADPmediator compounds that do not bind irreversibly to thiol groups in the active sites of intracellular dehydrogenase enzymes. Such mediator compounds avoid a common mode of enzyme inhibition. The mediators can therefore increase the stability and reliability of the electrical response in amperometric electrodes constructed from NAD- or NADP-dependent enzymes.

Biosensor electrode mediators for regeneration of cofactors

The disclosure provides for reagent compositions for biosensors comprising release polymers, methods of making such biosensors and films of reagent compositions comprising release polymers. The reagent compositions comprise a release polymer and an effective analyte detecting amount of an enzyme an enzyme cofactor and a redox compound capable of acting in a biosensor as (i) a redox mediator associated with a working electrode (ii) a redox couple associated with a reference electrode or (iii) the redox mediator associated with the working electrode and the redox couple for the reference electrode.

Biosensor coating composition and methods thereof

The present invention provides reagent compositions, and analyte measuring devices and methods that utilize the reagent compositions.

Biosensor having improved hematocrit and oxygen biases

Method of using a biosensor

Growing community of inventors

Abingdon, United Kingdom

Nigel John Forrow