Radiometer A/s

Radiometer Medical A/s

Niels-Henrik Jensen

Finn C Lundsgaard

Willy Andersen

In a method of photometric in vitro determination of the content of oxygen in a blood sample, a blood sample is transferred directly from an in vivo locality to an at least partially transparent sample container of a sampling device. The sample container has a measuring chamber containing a luminophor, the luminescence of which is quenched in the presence of oxygen. The luminophor is excited by irradiation with radiation from a radiation source. The luminescence emitted by the excited luminophor is detected by a radiation detector and the oxygen content determined on the basis of the radiation detected by the radiation detector. A sampling device and a system for photometric in vitro determination of oxygen in a blood sample are also described.

Method of photometric in vitro determination of the content of oxygen in

Method of photometric in vitro determination of a blood gas parameter in

A sampling device for photometric determination of the content of an analyte in a sample of whole blood has at least one measuring chamber having two wall parts, at least one wall part being locally transparent, at least one wall part being sufficiently deformable to facilitate displacement of the whole blood sample from the measuring chamber. The measuring chamber also contains a transparent body having a radiation transmission characteristic dependent upon the concentration of a predetermined analyte in the whole blood sample. The sample device is employed in an analyzer for photometric determination of the content of an analyte in a sample of whole blood and in a method of photometric in vitro determination of the content of an analyte in a sample of whole blood. In the method, a sample of whole blood is transferred directly from an in vivo locality to the sampling device, the measuring chamber therein is deformed in a controlled manner to substantially drain the whole blood from the measuring chamber, radiation is transmitted through the substantially drained measuring chamber, detected, and the analyte content determined.

Method of photometric in vitro determination of the content of an

The concentration of molecular oxygen in a sample is determined by exciting oxygen molecules of the sample from the electronic ground state to the excited .sup.1 .DELTA..sub.g state (excited singlet state), measuring a 1270-nm luminescence characteristic of the excited oxygen molecules (singlet oxygen) and correlating the luminescence characteristic measured with the concentration of molecular oxygen in the sample. Normally, the 1270-nm luminescence characteristic is the 1270-nm luminescence intensity. Preferably, the oxygen molecules are excited by being subjected to diffusion contact with a sensitizer such as a porphyrin or a porphyrin-related compound, e.g. a transition metal complex of a porphyrin. The sensitizer is brought into an oxygen-exciting electronic state by absorption of electromagnetic radiation such as light, the oxygen-exciting electronic state optionally being adapted to the measuring system employed by means of a quencher such as a substituted polyene. The sensitizer may be present in an organic solvent or a polymer such as polyvinylchloride. The oxygen-containing sample is preferably a sample of biological origin such as a blood sample.

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Farum, Denmark

Niels-Henrik Jensen