Promega Corporation

Other

Nidhi Nath

Poncho Meisenheimer

Keith V Wood

Thomas A Kirkland

Mary P Hall

Thomas Machleidt

Matthew B Robers

Melanie Dart

Lance P Encell

Monika G Wood

Brock Binkowski

Said A Goueli

James Unch

Andrew S Dixon

Hicham Zegzouti

Marie Schwinn

Subhanjan Mondal

Dileep K Pulukkunat

Marjeta Urh

Dieter Klaubert

Paul Otto

Rachel Friedman Ohana

Carolyn Woodroofe Hitko

Harry Tetsuo Uyeda

Robin Hurst

Wenhui Zhou

Ji Zhu

Rod Flemming

Hui Wang

Joel R Walker

Roderick G Flemming

Virginia E Kincaid

Becky Godat

Brock F Binkowski

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Activation of bioluminescence by structural complementation

Activation of bioluminescene by structural complementation

Provided herein are compositions, kits, and methods for performing analyte detection immunoassays.

Analyte detection immunoassay

Provided herein are sulfo n-hydroxysuccimidyl ester (sulfo-SE) linked peptides, methods of synthesis thereof, and methods of using such peptides for labeling of biomolecules. In particular, peptides comprising non-alkyl group such as serine, threonine, cysteine, tyrosine, glutamic acid, and aspartic acid are stably modified (e.g., without autoreactivity) with a sulfo-SE group and used to label or otherwise modify biomolecules.

Reactive peptide labeling

The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Compositions and methods for capture of cellular targets of bioactive agents

Compounds that may inhibit-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Functionalized NANOLUC inhibitors

The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Growing community of inventors

Madison, WI, United States of America

Nidhi Nath