Institut Pasteur

Centre National De La Recherche Scientifique

Nicole Guiso

Pascale Gueirard

Claude Leclerc

Daniel Ladant

Peter Sebo

Cecile Bauche

Cécile Bauche

Catherine Fayolle

Fotini Betsou

Mohammed El-Azami El-Idrissi

Pierre Guermonprez

Nadia Khelef

The invention relates to a novel use of aadenylcyclase toxin in the manufacturing of vectors for targeting in vivo a molecule of interest, specifically to CD11b expressing cells. The invention also relates to an immunogenic composition that primes immune responses, to pharmaceutical compositions and to a new vector for molecule delivery to CD11b expressing cells.

Vectors for molecule delivery to CD11b expressing cells

Purified nucleic acids comprising a nucleotide sequence encoding an immunogenic polypeptide of adenyl cyclase-haemolysin (AC-Hly), which induces formation of protective antibodies against an infection by a bacteria selected from the group consisting of, andwhen the nucleic acid or polypeptide is administered to a human or animal host. The nucleic acids are useful, for example, to induce a protective immune response in a host against infection by a bacteria selected from the group consisting of, and

Nucleic acids encoding protective epitopes of adenyl cyclase-haemolysin (AC-Hly)

The invention relates to an immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (AC-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain ofchosen fromorand in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain ofchosen fromoror a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.

Acellular antibordetella vaccine

This invention relates to an immunogenic composition comprising a bacterial extract containing at least one expression products of the vrg genes of a strain ofchosen from, or. The invention also relates to a method for producing the extract, comprising culturing the bacteria on blood medium to obtain isolated nonhemolytic colonies; inoculating cells of one or more colonies in liquid medium to give a suspension of cells; separating the cells from the liquid medium after culture; suspending the separated cells in a buffer comprising urea for at least an amount of time sufficient to form a bacterial lysate; and separating intact cells and insoluble material from soluble material, wherein the extract comprises the soluble material.

[object Object]

The invention relates to an immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (AC-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain of Bordetella chosen from or and in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain of Bordetella chosen from or or a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.

The subject of the invention is amino acid sequences of the AC-Hly from,and/or,carrying epitopes capable of inducing a protective immune response against infection by Bordetella. The subject of the invention is antibodies, especially monoclonal antibodies, directed against these epitopes.

Protective epitopes of adenyl cyclase-haemolysin (AC-Hly), their application to the treatment or to the prevention of bordetella infections

An immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (Ac-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, and in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, or a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.

Bordetella pertussis and Bordetella parapertussis strains

The invention relates to an immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (AC-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, and in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, or a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.

Growing community of inventors

Paris, France

Nicole Guiso