Merial Limited

Top Industrie S.a.s.

Boehringer Ingelheim Animal Heatlh USA Inc.

Nicolas Pierre Yves Carboulec

Laurent Bernard Fischer

Fabien Lux

Lionel Gerentes

Rene Labatut

Gildas Merian

The present invention encompasses vaccines or compositions comprising the chimeric KSAC protein that possesses immunogenic and protective properties, and methods of use including administering to an animal the antigenic KSAC protein thereof to protect animals. The invention also encompasses methods for making and producing the soluble, disaggregated, refolded or active proteins from inclusion bodies produced from prokaryotes or eukaryotes.

Expression of chimeric KSAC protein and method of producing soluble proteins by high pressure

The invention relates to hyperbaric devices for inactivating microorganisms and viruses while retaining their immunogenicity and for making and producing the soluble, disaggregated, refolded or active immunogenic or therapeutic proteins from inclusion bodies produced from prokaryotes or eukaryotes. The invention encompasses hyperbaric methods for inactivating pathogenic organisms, and methods for producing vaccine compositions using the inactivated pathogens. The hyperbarically inactivated microorganisms are safer and more immunogenic than chemically inactivated microorganisms. Similarly, the solubilized proteins have superior properties compared to more heavily aggregated proteins, including reduced non-specific immune reactions.

Hyperbaric device and methods for producing inactivated vaccines and for refolding/solubilizing recombinant proteins

A process for the culture of Leptospires in a protein-free culture medium and without any fatty acid source in the culture medium, wherein a fatty acid source is fed continuously or intermittently.

Leptospirosis culture process

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Saint Goueno, France

Nicolas Pierre Yves Carboulec