Roche Molecular Systems, Inc.

Other

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Nicolas Newton

Stephen Gordon Will

Thomas W Myers

Amar Gupta

Alison Tsan

Nancy J Schoenbrunner

Dirk Zimmermann

Heike Wilts

Ellen H Fiss

Joseph San Filippo

Hermann Leying

Eberhard Russmann

Xiaoning Wu

Karen K Y Young

Edward Soh Smith

Meike Eickhoff

Christopher D Newhouse

John Niemiec

Niclas Hitziger

Lori Steiner

Edward S Smith

Kevin Janssen

John T Niemiec

Anke Mussmann

Stephen G Will

Ning Kyle Lu

Karen Kwok Ying Young

Andreas WÖLFELSCHNEIDER

Christopher Newhouse

Nancy Schoenbrunner

A method of simultaneously amplifying genotypes 1, 2, 3 and/or 4 of HEV is disclosed comprising amplifying the genotypes 1, 2, 3 and/or 4 of HEV with one single none-degenerate forward primer partially overlapping the 5'UTR region of HEV and at least one reverse primer. Also disclosed are related methods comprising a probe, and kits for the detection of genotypes 1, 2, 3 and/or 4 of HEV.

HEV Assay

Methods and oligonucleotides are provided for detecting an internal control nucleic acid for qualitative and/or quantitative purposes.

Oligonucleotides for controlling amplification of nucleic acids

HEV assay

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3'-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3&#x2032;-terminal nucleotide.

Allele-specific amplification

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, having an internally-placed selective nucleotide complementary to only one variant of the target sequence wherein the allele-specific oligonucleotide is extended by a nucleic acid polymerase predominantly or exclusively when hybridized to the variant of the target sequence for which it has said complementary selective nucleotide.

Allele-specific amplification of nucleic acids

The present invention provides a method for the amplification of at least a first and a second target nucleic acid that may be present in a fluid sample. The invention further provides a kit and an analytical system for carrying out said amplification.

Generic PCR

The present invention relates to a method, oligonucleotides, reaction mixtures and kits for the selective amplification of a messenger RNA target comprising an exon-exon junction, using an oligonucleotide that comprises at least one nucleotide modified at the exocyclic amino group.

Preferential amplification of mRNA over DNA using chemically modified primers

Methods and compositions for amplification of a target sequence by suppressing amplification of related sequences are provided.

Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3&#x2032; nuclease activity

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Oakland, CA, United States of America

Nicolas Newton