Centre National De La Recherche Scientifique

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Universite Claude Bernard – Lyon1

Institut National Des Sciences Appliquees De Lyon

Ecole Centrale De Lyon

Cpe Lyon Formation Continue Et Recherche

Avalun

Nicolas Gaignebet

Mathieu Dupoy

Maryse Fournier

Taha Benyattou

Lotfi Berguiga

Patrick Pouteau

Cécile Jamois

Jean-Marc Fedeli

Thomas Gehin

The invention relates to a method for analysing a sample (), the sample being capable of comprising an analyte, the sample extending on a resonant support ((, the resonant support having a surface on which a plurality of photonic crystals extend (), separated from one another, such that the sample extends between a light source () and the photonic crystals, the photonic crystals being such that: —a resonance wavelength (λ) is associated with each photonic crystal () addressing the analyte, wherein the wavelengths of the photonic crystals define a resonance spectral band (Δλ), extending between 2 μm and 20 μm; —the transmission or the reflection of light by each photonic crystal addressing the analyte is maximum at the associated resonance wavelength; the method comprises the following steps: a) illuminating the support by the light source, the light source emitting an illumination lightwave (), defining an illumination spectral band (Δλ) which at least partially covers the resonance spectral band (Δλ), such that a plurality of photonic crystals are simultaneously illuminated; b) acquiring an image (I) of the resonant support, then determining the presence of the analyte in the sample from the image.

Method and device for analysing a sample using a resonant support, illuminated by infrared radiation

A method for analysing a sample uses a resonant support having a surface on which a plurality of separated photonic crystals extends. At least two crystals are configured to capture the same analyte. A resonance wavelength associated with each crystal varies with an amount of analyte in contact with the crystal. The wavelengths define a resonance spectral band between 200-1500 nm. The transmission/reflection of the light is maximum at an associated resonance wavelength. The method includes: illuminating the support in the resonance spectral band, the intensity of the lamination being variable in band; acquiring a measurement image using an image sensor, the image having different regions-of-interest each optically coupled to a photonic crystal; using a reference image representative of an image acquired by the image sensor, when the support is illuminated in the resonance spectral band in a reference configuration; and comparing the measurement image with the reference image.

Method and device for analysing a sample, implementing a resonant support

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La Tour d'Aigues, France

Nicolas Gaignebet