Qiagen Gmbh

Nicola Scholle

Thorsten Singer

Dominic O'Neil

Margit Hiesinger

Peter Grünefeld

Tanya Sperling

Tanja Sperling

The present invention provides method for isolating DNA molecules having a size above a certain cut-off value from a DNA containing sample, comprising a) contacting the sample with a binding buffer which comprises a chaotropic agent and a buffering agent to provide a binding mixture and binding DNA molecules having a size above the cut-off value to a binding matrix which has a silicon containing surface, wherein the cut-off value is determined by the pH value of the binding mixture; b) separating the bound DNA from the remaining sample; c) optionally washing the bound DNA; and d) optionally eluting the bound DNA from the binding matrix. Said method allows the size selective purification of DNA molecules.

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Hilden, Germany

Nicola Scholle