Universiteit Gent

Vib Vzw

Research Corporation Technologies, Inc.

Nico Lm Callewaert

Roland Henri Contreras

Steven Cj Geysens

The present invention provides genetically engineered strains ofcapable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an &#x3b1;-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains ofare also provided.

Protein glycosylation modification in methylotrophic yeast

The invention provides methods and kits to detect liver fibrosis or a change in the gradation of liver fibrosis in mammals. The diagnostic marker is based on the profiling and identification of diagnostic carbohydrates present in a body fluid such as blood serum.

Growing community of inventors

Lichtervelde, Belgium

Nico Lm Callewaert