Arizona State University

Nicholas Stephanopoulos

Minghui Liu

Yang Xu

Alexander A Green

Neal W T Woodbury

Chris Diehnelt

Matthew Gilliam

Crystal Willingham

Kirstie Swingle

Sarah Stabenfeldt

Briana Martinez

Kendall Lundgreen

Amanda Witten

A photocaged DNA nano-tweezer and methods of using said photocaged DNA nano-tweezer are described. In particular, provided herein is a DNA nano-tweezer comprising a hairpin with a single-stranded loop that comprises a first arm and a second arm; and a trigger strand complementary to the single-stranded loop and comprising at least one photocaged residue with a protecting group.

Reconfigurable DNA nano-tweezer

Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.

Tunable nanoscale cages from self-assembling DNA and protein building blocks

A unique pipeline is employed for biomarker discovery that entailed domain antibody phage display, next generation sequencing analysis, and nanotechnology strategies to generate antibody mimetics are disclosed. Also disclosed are the temporal biomarkers of traumatic brain injury and their methods of use. In some embodiments, the temporal biomarkers are synthetic peptides comprising the HCDR3 sequences identified using the disclosed pipeline. In some aspects, the synthetic peptides have less than 30 amino acid residues and comprise a biotin scaffold that is linked to the HCDR3 sequences.

Temporal traumatic brain injury biomarkers and methods of use thereof

Integrated diagnostic devices comprising peptide-DNA conjugates for analyte detection, an embedded biomolecular computing system for sample analysis, and a layered device architecture are provided herein. In particular, provided herein are devices comprising a layered architecture that enables diagnostic reagents, sample components, and reaction products to flow through the system with minimal user intervention.

Integrated diagnostic devices having embedded biomolecular computing systems and uses thereof

Growing community of inventors

Scottsdale, AZ, United States of America

Nicholas Stephanopoulos