Boehringer Mannheim Gmbh

Nicholas R Hoyle

Gunter Pappert

Ulrich Neumann

Christa Hubner-Parajsz

Michael Grol

Volker Klemt

Ursula Giesen

Gunter Muller

Helmut Lenz

Norbert Franken

Helmut Rehner

Peter Stegmuller

Peter Bialk

Albert Kunst

Electrochemiluminesence assay

Electrochemiluminescence assay

A method for making an monoclonal antibody specific for vitamin B12 by immunizing mice with vitamin B12-b-acid and isolating hybridomas that produce monoclonal antibodies which have an affinity constant for B12 greater than 5.times.10.sup.9 l/mol is disclosed.

Method and monoclonal antibodies for vitamin B12 determination

The present invention provides a storable POD conjugate solution, characterised by a pH value of 5.0 to 6.5, adjusted by means of an organic buffer substance with a concentration of 40 to 100 mmol/l, and 0.05 to 1.0 mol/l magnesium aspartate.

Storable protein solution

For the determination of vitamin B12 a sample solution is incubated with at least two receptors R.sub.1 and R.sub.2 of which R.sub.1 mediates the binding to the solid phase and R.sub.2 is labelled, whereby a receptor is used as one of the receptors R.sub.1 or R.sub.2 which contains a monoclonal antibody capable of specific binding to B12 that has an affinity constant of at least 5.times.10.sup.9 l/mol and a receptor is used as the other receptor R.sub.1 or R.sub.2 which contains B12 or an analogue thereof, the two phases are separated and the label is measured in one of the two phases.

A standard solution containing thyroxine-binding globulin (TBG) and thyroxine or triiodothyronine dissolved in a buffer solution is used for calibration in a method for the determination of thyroxine (T.sub.4) or triiodothyronine (T.sub.3) in serum, wherein both human and bovine TBG can be used.

Method and standard solution for the determination of thyroxine

A process for the dissolving off of an analyte bound to a binding protein by breakdown of the binding protein using a cleaving agent which cleaves --S--S-- groups into SH groups in the alkaline range, wherein a 1,2-dithiolan-3-carboxylic acid of the formula: ##STR1## is used as the --S--S-- group cleaving agent in which n is a whole number of from 1 to 8. The process is used for preparing samples for analytical or investigational processes for the determination of a liberated analyte and especially for immunoassays for vitamins such as vitamin B.sub.12, steroids such as testosterone or for thyroid hormones such as T.sub.3 /T.sub.4.

Process for liberating an analyte from its binding protein

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Tutzing, Germany

Nicholas R Hoyle