Upside Foods, Inc.

Other

University of Missouri

Memphis Meats, Inc.

Nicholas J Genovese

Uma S Valeti

Matthew Leung

Kathleen Carswell

Asha Godbole

George C Engelmayr, Jr

George C Engelmayr

Eric N Schulze

Danielle Nicole Desmet

Napat Tandikul

R Michael Roberts

Bhanu Prakash V L Telugu

Meri Teresa Firpo

Daphné Dambournet

Described herein are novel systems and methods for biomanufacturing, such as tissue cultivation. In some variations the systems and methods may comprise at least one porous substrate.

Methods for cultivating tissue on porous substrates

Provided herein are methods to increase the culture density and/or thickness of a cellular biomass in a cultivation infrastructure, to improve the culture of cells in the absence of serum in a cultivation infrastructure, and to promote anchorage-independent growth of a cellular biomass in a cultivation infrastructure. The methods comprise inhibiting the HIPPO signaling pathway, for example, by activating YAP1, activating TAZ, and/or inhibiting MOB1, LATS1 kinase, LATS2 kinase, WW45, MST1 kinase, and/or MST2 kinase in the cellular biomass. In some embodiments, the cellular biomass is harvested from the cultivation infrastructure for the formulation of cell-based food products or ingredients, such as animal meat manufactured from cells in an ex vivo process or for therapeutic applications such as organ or tissue transplantation or grafting.

Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure

Systems and methods for cultivating tissue on porous substrates

Provided herein are compositions and methods to make and use engineered cells, for the purpose of increasing the cell density of a culture comprising metazoan cells and for the production of a cultured edible product.

Compositions and methods for increasing the efficiency of cell cultures used for food production

The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.

Methods for preparing a comestible meat product

Substrates for preparing a comestible meat product

Apparatuses and methods for preparing a comestible meat product

The present disclosure relates to methods for enhancing cultured meat production, such as livestock-autonomous meat production. In certain aspects, the meat is any metazoan tissue or cell-derived comestible product intended for use as a comestible food or nutritional component by humans, companion animals, domesticated or captive animals whose carcasses are intended for comestible use, service animals, conserved animal species, animals used for experimental purposes, or cell cultures.

Method for scalable skeletal muscle lineage specification and cultivation

A portable anchoring device having a receiving sleeve which is a cylindrical elongated tube having a throughbore therein. The receiving sleeve houses a filling chamber , filling cap , and the securing ring . The filling chamber provides the means by which the portable anchoring device may add more stability to its anchoring function and is equipped with an inlet cap and an outlet cap . The retaining ring is located at the base end of the receiving sleeve and provides the portable anchoring device with the means for securing a plurality of portable anchoring devices to each other. The retaining ring also provides the means for securing a tent pole or gazebo leg to the portable anchoring device. The base support element is composed of two, semi-circle shaped units that provide the portable anchoring device with its foundation and anchorage support. The base support element also houses the emptying cap with emptying orifice and a portion of the filling chamber . Also the base support element provides the portable anchoring device with stability when the filling chamber is filled or empty.

Portable anchoring boot

Growing community of inventors

Hayward, CA, United States of America

Nicholas J Genovese