Sphere Fluidics Limited

Nicholas Dayrell-Armes

Frank F Craig

Marian Rehak

David Holmes

Robert Salter

William Whitley

Dimitris Josephides

Raphael Ruis

Sinan Gokkaya

A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.

Droplet processing methods and systems

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Cambridgeshire, United Kingdom

Nicholas Dayrell-Armes