Illumina Corporation

Epicentre Technologies Corporation

Nicholas Caruccio

Jerome J Jendrisak

Gary A Dahl

Haiying Li Grunenwald

Kevin L Gunderson

Frank J Steemers

Sasan Amini

Fiona Kaper

Igor Yu Goryshin

Mark A Maffitt

Natasha Pignatelli

Thomas Royce

Casey Turk

Reza Kahlor

Reza Kalhor

The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5'-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called 'next generation sequencing').

Transposon end compositions and methods for modifying nucleic acids

Artificial transposon sequences having code tags and target nucleic acids containing such sequences. Methods for making artificial transposons and for using their properties to analyze target nucleic acids.

Linking sequence reads using paired code tags

The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5'-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5&#x2032;- and 3&#x2032;-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5&#x2032;-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3&#x2032;-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5&#x2032;- and 3&#x2032;-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing so-called 'next generation sequencing').

The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5'-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5&#x2032;- and 3&#x2032;-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5&#x2032;-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3&#x2032;-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5&#x2032;- and 3&#x2032;-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called 'next generation sequencing).

Compositions of transposome complexes for generating DNA fragments with specific 5'- and 3&#x2032;-tags. Kits for generating libraries for sequencing, with transposome complexes, enzymes, oligonucleotides or other components.

The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5'-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5&#x2032;- and 3&#x2032;-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5&#x2032;-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3&#x2032;-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5&#x2032;- and 3&#x2032;-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called 'next-generation sequencing.).

The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5'-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5&#x2032;- and 3&#x2032;-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5&#x2032;-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3&#x2032;-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5&#x2032;- and 3&#x2032;-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called 'next-generation sequencing.)

Growing community of inventors

Madison, WI, United States of America

Nicholas Caruccio