The University of North Carolina at Chapel Hill

Cell Microsystems, Inc.

Nicholas C Trotta

Nancy L Allbritton

Steven C Gebhart

Christopher E Sims

Yuli Wang

Peter Joseph Attayek

Nicholas C Dobes

Robert W McClellan

Paul Michael Armistead

Robert W Mcclellan

Embodiments of the disclosed subject matter provide an automated method and system to isolate and collect cells using computerized analysis of images of cells and their surroundings obtained from a digital imaging device or system. Embodiments of the disclosed subject matter make use of a 'microwell array,' which can comprise a formed, elastomeric grid of indentations or “wells.” Many, most, or all of the wells in a microwell array can contain a releasable, microfabricated element, which can be referred to as a “raft.” Embodiments of the disclosed subject matter provide a system and method for cell collection that includes computerized identification and collection of rafts with isolated single cells or a specific group or groups of cells, eliminating the need for continuous human identification and selection.

Automated collection of a specified number of cells

An array of magnetic or paramagnetic micro-elements comprised of polysilsesquioxane is described having ultra-low-autofluorescence and other optical properties to improve microscopic imaging of cells or other objects present on the array. These materials are also amenable to chemical modification allowing surface attachment of affinity capture moieties or chemical reporters for selective binding or analysis of cells, macromolecules or other targets.

Array of micro-elements for high resolution and high content imaging and sorting of cells

Growing community of inventors

Durham, NC, United States of America

Nicholas C Trotta