Abic Biological Laboratories Teva Ltd.

University of Technology Sydney

Abic Biological Laboratories Ltd.

Nicholas C Smith

Michael Wallach

Sabina I Belli

David Witcombe

The present invention provides the recombinant cloning and sequencing of two of the majorgametocyte antigens having molecular weights of 56 and 82 kDa and the expression of these recombinant antigens in anexpression system using the plasmid pTrcHis. The subject invention also provides a vaccine against coccidiosis comprising the recombinant 56 kDa or 82 kDa antigen. The subject invention also provides two 30 kDa proteins and three 14 kDa proteins fromgametocytes having at the N-terminal end the amino acid sequence described herein. The subject invention also provides a vaccine against coccidiosis comprising the recombinant 56 kDa or 82 kDa antigen and any of the aforementioned proteins. Additionally, the subject invention also provides a method of immunizing a subject against infection byor, or a microorganism expressing an immunologically cross-reactive antigen, comprising the step of administering to the subject any of the aforementioned vaccines.

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The present invention provides an isolated nucleic acid comprising a nucleotide sequence encoding a 250 kDa polypeptide from Sporozoites/Merozoites of, or encoding a homolog of the polypeptide, or a complement of the nucleic acid and a method of producing a recombinant 250 kDa polypeptide of the same. The present invention also provides an isolated nucleic acid comprising a nucleotide sequence encoding an immunodominant portion of a 250 kDa polypeptide from Sporozoites/Merozoites ofhaving the amino acid sequence described herein, or encoding a homolog of the polypeptide, or a complement of the nucleic acid. The subject invention also provides a vaccine againstor, or a microorganism expressing an immunologically cross-reactive antigen, comprising the isolated nucleic acid comprising a nucleotide sequence encoding a 250 kDa polypeptide from Sporozoites/Merozoites ofor the immunodominant portion of a 250 kDa polypeptide from Sporozoites/Merozoites of, or encoding a homolog of the polypeptide, or a complement of the nucleic acid which may also contain a 56 kDa, 82 kDa or 230 kDa protein isolated from the gametocytes ofand a method of immunizing a subject against infection byor, or a microorganism expressing an immunologically cross-reactive antigen, comprising the step of administering to the subject any of the aforementioned vaccines.

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Roseville, Australia

Nicholas C Smith