Owl Biomedical, Inc.

Miltenyi Biotec B.v. Co., Kg

Nicholas C Martinez

John Stuart Foster

Kevin Shields

Stefan Miltenyi

Ralf-Peter Peters

Eiad Kabaha

Daryl Grummitt

Kimberly L Turner

Jaquelin K Spong

Christian Peth

Markus Nagel

Mehran Hoonejani

Jan Boddenberg

Kamala Qalandar

Martin BÜSCHER

A MEMS-based cell sorting system is disclosed, which uses a novel combination of features to accomplish the cell sorting in the microfabricated channels housed in a disposable cartridge. The MEMS-based cell sorting system may include a microfabricated cell sorting valve that is responsive to an applied magnetic field. The MEMS-based cell sorting system may further include an electromagnet that generates a magnetic field to actuate the microfabricated cell sorting valve. The electromagnet may have a design which allows it to create a very localized magnetic field while having adequate thermal properties to operate reliably.

Cell sorting system using electromagnetic solenoid

A MEMS-based cell sorter is disclosed, which uses a novel combination of features to accomplish the cell sorting using a microfabricated cell sorting valve housed in a disposable cartridge. The features include an interposer that provides fluid communication between the microfluidic passages in the silicon substrate and a plurality of fluid reservoirs in the cartridge, including a sample, sort and waste reservoir The disposable cartridge may include other features that assist in the handling of small volumes of fluids, such as a siphon region in the sort reservoir and funnel-shaped regions in the sample and waste reservoirs. A mixing mechanism may be provided for stirring the contents of the sample reservoir.

Cell sorting system using microfabricated components

A particle separation system uses a MEMS-based, microfabricated particle manipulation device which has an inlet channel, output channels, and a movable member formed on a substrate to sort one or more target particle from a sample stream. The system may include an interposer that receives the sorted particle and dispenses a carrier fluid with it to form a liquid droplet containing the particle. The droplet may then be dispensed to a microtiter plate, such that each well in the titer plate may contain a single target particle. The system may be used to separate individual biological cells, such as T cells, B cells, stem cells, cancer cells and sperm cells for further manipulation.

MEMS-based single particle separation system

A particle manipulation system uses a MEMS-based, microfabricated particle manipulation device which has an inlet channel, output channels, and a movable member formed on a substrate. The movable member moves parallel to the fabrication plane, as does fluid flowing in the inlet channel. The movable member separates a target particle from the rest of the particles, diverting it into an output channel. However, at least one output channel is not parallel to the fabrication plane. The device may be used to separate a target particle from non-target material in a sample stream. The target particle may be, for example, a stem cell, zygote, a cancer cell, a T-cell, a component of blood, bacteria or DNA sample, for example. The particle manipulation system may also include a microfluidic structure which focuses the target particles in a particular portion of the inlet channel.

Particle manipulation system with out-of-plane channel and focusing element

Particle manipulation system with out-of-plane channel

A MEMS-based particle manipulation system which uses a particle manipulation stage and a sensor to detect when the sample volume is exhausted or nearly exhausted. The sensor sends a signal to a fluid control means that reverses the pressure between one of the output channels and the input channels, to keep the surfaces wet with a volume of the sample fluid. This volume can be maintained in the channel until an operator intervenes, or it can be repeatedly shuttled back and forth between the input channel and an output channel. By keeping the channels wet, material from the sample stream does not become adhered to the channel walls, which might otherwise irreversibly change or damage the device.

Particle manipulation system with stay-wet algorithm

A MEMS-based particle manipulation system which uses a particle manipulation stage and a plurality of laser interrogation regions. The laser interrogation regions may be used to assess the effectiveness or accuracy of the particle manipulation stage. In one exemplary embodiment, the particle manipulation stage is a microfabricated valve, which sorts a target particle from non-target particles in a fluid stream. The laser interrogation stages are disposed in the microfabricated fluid channels at the input and output of the valve. By reversing the flow from output to input, the same laser interrogation region may be used to perform the cytometry. The cytometry may be performed throughout the sorting process to optimize or control the sorting, or may be performed afterward to allow a multi-pass, sequential sort to be performed on the same sample.

Particle manipulation system with cytometric confirmation

Growing community of inventors

Santa Barbara, CA, United States of America

Nicholas C Martinez