Perfinity Biosciences, Inc.

Perfinity Bioscices, Inc.

Nicholas Brian Herold

Kevin Wayne Meyer

John Patrick O'Grady

Derrick Nathaniel Poe

Fred E Regnier

Robert Harold Ellis

Bob Harold Ellis

This disclosure provides a single reactor that accommodates an affinity selector to separate analytes of interest, and an enzyme reactor that digest the analyte to suitable peptides for mass spectrometry. The single reactor formats described herein accommodate workflows wherein separation precedes digestion as well as workflows wherein digestion precedes separation selection.

Single reactor for simplified sample preparation workflows

The disclosure provides an innovative immobilized enzyme reactor working system that includes improved reactor formats, heating element, optimum buffers, filtration apparatus and collection componentry for easy to use sample preparation. The system provides enhanced recovery, improved reproducibility and parallel processing capabilities capable of quantitatively processing hundreds of difficult to digest samples simultaneously in a shortened period of time.

Robust, easy to use immobilized enzyme reactors

A multi-dimensional method for simultaneously analyzing multiple analytes within a sample solution, comprising adding affinity selectors to a sample solution containing analytes to be measured, the affinity selectors having an affinity for one or more of the analytes within the sample solution; allowing immune complexes to form between the affinity selectors and the analytes; partially or totally resolving the formed immune complexes from non-analyte substances within the sample solution; dissociating the resolved immune complexes; separating the analytes and the affinity selectors of the dissociated immune complexes from one another by capturing the analytes through a surface adsorption process; transferring the captured analytes to a detection means; and resolving the analytes with the detection means in accordance with their mass-to-charge ratios.

Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis

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West Lafayette, IN, United States of America

Nicholas Brian Herold