Bowling Green State University

Neocles Leontis

Kirill Andreevich Afonin

The invention includes RNA complexes comprising at least three monomeric units of an RNA molecule, each monomeric unit comprising an RNA polymer having first and second helical domains that have respective first and second binding sites, wherein the first binding sites are adapted to binding to one another and are not adapted to bind to the second binding sites, and the second binding sites are adapted to binding to one another and are not adapted to bind to the first binding sites; such that the at least three monomeric units are adapted to self-assemble by forming pairs of cognate interactions and so as to form the RNA complex in a circular closed complex. The invention also includes derivatives of these complexes including aptamers, and analytical methods and devices using same.

RNA complexes, methods of their production and sensors and analytical methods involving same

RNA complexes, method of their production and sensors and analytical methods involving same

The present invention pertains to the use of paranemic crossover motifs for RNA self-assembly, and the use of those self-assembled complexes to carry out analytical methods, and as a basis for the creation of sensors. The invention includes RNA monomeric units comprising an RNA having a binding domain that is adapted to self-assemble paranemically so as to form an RNA complex with another RNA, the binding domain of the RNA monomeric unit comprising an aptameric portion bound to a fluorophore. The invention also includes RNA complexes comprising a target RNA molecule and at least one of these monomeric units which self-assemble paranemically so as to form the RNA complex comprising an aptameric portion. The invention also includes derivatives of these complexes including aptamers, and analytical methods and devices using same.

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Bowling Green, OH, United States of America

Neocles Leontis