National Health Research Institutes

Taipei Medical University

Development Center for Biotechnology

Dcb-usa LLC

Neng-Yao Shih

Ko-Jiunn Liu

Yi-Yuan Yang

Sy-Jye Leu

I-Jen Huang

Yuan-Soon Ho

Li-Tzong Chen

Ta-Tung Yuan

Shih-Chong Tsai

Shih-Chi Tseng

Yu-Ching Lee

Chi-Hsin Lee

Wen-Shyang Hsieh

Hsueh-Hsia Wu

Chao-Yang Huang

Jiann-Shiun Lai

Ying-Yung Lok

Shih-Lan Hsu

Chia-Cheng Wu

Chung-Hsiun Wu

Ya-Wei Tsai

Hsin-Yun Wang

Suparat Charoenfuprasert

Shih-Yi Huang

Chih-Yung Hu

Yu-Jia Chang

Gee-Chen Chang

Yueh-Lun Lee

Wen-chun Hung

Yun-chang Chen

Kuan-chung Hsiao

Mei-Ru Chen

Ching-Yu Lin

San-tai Shen

Mingl Chang

Yung-Luen Shih

Bor-Yu Tsai

Yung-Leun Shih

Po-Yin Lin

Hsien-Yu Tsai

Wei-Tso Chia

Shyi-Jou Chen

An isolated antibody, comprising heavy chain complementary determining regions CDR1, CDR2, and CDR3 from a heavy chain variable region sequence having SEQ ID NO: 1 or 3; light chain complementary determining regions CDR1, CDR2, and CDR3 from a light chain variable region sequence having SEQ ID NO: 2 or 4; wherein the antibody binds specifically to both vascular endothelial growth factor receptor-2 (VEGFR2) and vascular endothelial growth factor receptor-3 (VEGFR3).

Dual-function antibodies targeting VEGFR2 and VEGFR3

An antibody, or an antigen-binding fragment there, binding human ENO1 (GenBank: AAH506421.1) is provided. Methods for treating an ENO1 protein-related disease or disorder, inhibiting cancer invasion and diagnosis of cancer are also provided.

Antibodies specific to alpha-enolase and uses thereof

A method for treating an inflammatory disease or an immune disorder includes administering to a subject in need of such treatment an antagonist against ENO1. The antagonist binds ENO1 and inhibits ENO1 plasminogen receptor activity. The antagonist may be an anti-human ENO1 antibody, or an scFv, Fab, or F(ab)fragment thereof, that specifically binds to human ENO1 (GenBank: AAH50642.1) for the treatment of an inflammatory disease or an immune disorder, which may be multiple sclerosis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, systemic Lupus erythematosus, chronic obstructive pulmonary disease (COPD), asthma, allergy, psoriasis, type 1 diabetes mellitus, artherosclerosis or osteoporosis.

Alpha-enolase specific antibodies and method of use in immune diseases

A humanized antibody, or a binding fragment thereof, wherein the humanized antibody binds human ENO1 (GenBank: AAH50642.1), wherein the antibody comprises a light chain variable region (VL) domain comprising a CDR1 having the amino acid sequence LCDR1 (RASENIYSYLT; SEQ ID NO: 6) and a CDR2 having the amino acid sequence LCDR2 (NAKTLPE; SEQ ID NO: 7) and a CDR3 having the amino acid sequence LCDR3 (QHHYGTPYT; SEQ ID NO: 8) and an antibody heavy chain variable region (VH) domain comprising a CDR1 having the amino acid sequence HCDR1 (GYTFTSCVMN; SEQ ID NO: 3), a CDR2 having the amino acid sequence HCDR2 (YINPYNDGTKYNEKFKG; SEQ ID NO: 4) and a CDR3 having the amino acid sequence HCDR3 (EGFYYGNFDN; SEQ ID NO: 5), wherein framework regions in the light chain variable region (VL) domain and the heavy chain variable region (VH) domain comprise amino acid sequences from a human immunoglobulin.

Humanized alpha-enolase specific antibodies and methods of uses in cancer therapy

The present invention discloses an anti-human alpha-enolase (ENO1) antibody, which can bind the peptides, comprising amino-acid sequence 296FD Q D D W G A W Q K F TA309 (SEQ ID: #9) and/or 326K R I A K A V N EK S336 (SEQ ID: #10) of human ENO1 protein (GenBank: AAH50642.1), has a favorable binding activity (the binding affinity is around 2.19&#xd7;10-10 mol/L) and a remarkable capability to inhibit the cell invasion and tumor metastasis of a varied of tumors. The recognized peptides and antibody of the invention are useful for diagnosis, prognosis, and treatment of cancers that have been reported to express cell-surface ENO1 such as including lung, breast, pancreas, liver, colorectal, prostate cancers and solid tumors.

Alpha-enolase specific antibodies and methods of uses in cancer therapy

The invention provides an isolated antigen polypeptide that can be expressed in a subject with ovarian cancer. Also provided is the diagnosis of ovarian cancer by using the antigen polypeptide of the invention and the prevention and/or treatment of ovarian cancer by suppressing the gene of the antigen polypeptide of the invention.

Method for the diagnosis of the presence of an ovarian cancer

The invention relates to antibodies against &#x3b1;-enolase I, their pharmaceutical compositions and diagnosis and treatment uses. Particularly, the invention provides polyclonal anti-&#x3b1;-enolase I antibodies and monoclonal single-chain variable fragment (scFv) anti-&#x3b1;-enolase antibodies, pharmaceutical compositions containing the same and their uses in uses in diagnosis and treatment of cancers, autoimmune disorders, ischemia and bacterial infection.

Anti-&#x3b1;-enolase I antibodies for diagnosis and treatment of &#x3b1;-enolase I-associated diseases

The present invention provides a method for the treatment and/or prevention of bacterial infection caused by Enterobacteriaceae bacteria in central nervous system and/or peripheral blood circulation in a mammal by administering effective amount of outer membrane protein A (OmpA) or its derivatives to a mammal. Also provided are a method for vaccinating a mammal to produce an antibody against bacterial infection caused by Enterobacteriaceae family in central nervous system and/or peripheral blood circulation and a method of detecting or diagnosing bacterial infections caused by Enterobacteriaceae family in central nervous system and/or peripheral blood circulation in a mammal. An antibody and a kit on the basis of the vaccinating method and detecting/diagnosing method are also provided.

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Antigen polypeptide for the diagnosis and/or treatment of ovarian cancer

The present invention provides a method for the treatment and/or prevention of bacterial infection caused byand other gram-negative bacteria in central nervous system and/or peripheral blood circulation in a mammal by administering effective amount of outer membrane protein A (OmpA) or its derivatives to a mammal. Also provided are a method for vaccinating a mammal to produce an antibody against bacterial infection caused byfamily in central nervous system and/or peripheral blood circulation and a method of detecting or diagnosing bacterial infections caused byfamily in central nervous system and/or peripheral blood circulation in a mammal.

This invention relates to a method of monitoring cancer development by determining the abundance of alpha-enolase protein wherein increased abundance is an indication of the severity of cancer. In another embodiment, the invention relates to a method of detecting cancer malignancy by determining the abundance of alpha-enolase antibodies in a sample wherein low levels of such antibodies indicates the malignancy of cancer. Also provided is a method of suppressing tumor growth by inducing the anti-ENO1 immune response.

Alpha-enolase specific antibody and method of use

The present invention provides a method for the treatment and/or prevention of bacterial infection in central nervous system and/or peripheral blood circulation in a mammal by administering effective amount of outer membrane protein A (OmpA) or its derivatives to a mammal. Also provided is an antibody binding to OmpA that can assay OmpA levels in a biological sample and detect or diagnose bacterial infection in central nervous system and/or peripheral blood circulation.

Use of outer membrane protein a in treatment/prevention/diagnosis of bacterial infection in central nervous system and/or peripheral blood circulation

Growing community of inventors

Miaoli County, Taiwan

Neng-Yao Shih